Reliability of human cortical organoid generation

Nat Methods. 2019 Jan;16(1):75-78. doi: 10.1038/s41592-018-0255-0. Epub 2018 Dec 20.


The differentiation of pluripotent stem cells in three-dimensional cultures can recapitulate key aspects of brain development, but protocols are prone to variable results. Here we differentiated multiple human pluripotent stem cell lines for over 100 d using our previously developed approach to generate brain-region-specific organoids called cortical spheroids and, using several assays, found that spheroid generation was highly reliable and consistent. We anticipate the use of this approach for large-scale differentiation experiments and disease modeling.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cell Line
  • Humans
  • Organoids / growth & development*
  • Pluripotent Stem Cells / cytology
  • Prosencephalon / physiology
  • Reproducibility of Results
  • Sequence Analysis, RNA
  • Single-Cell Analysis / methods
  • Tissue Engineering*