Standardization of the HbA2 Assay

EJIFCC. 2018 Dec 5;29(4):298-302. eCollection 2018 Dec.


Background: A project for the standardization of HbA2 was launched by the IFCC back in 2004.

Materials and methods: In this work we report on the state-of-the-art of the project on standardization of HbA2. Data obtained from various EQAS studies, and from previous experimental evaluations, are presented.

Results: We have proven that biases between various commercial methods are still currently significant. We have also shown that calibration by commutable control materials may halve the inter-method variability.

Conclusions: The foundation of the reference system for HbA2, together with a brief preliminary presentation of the proposed primary reference measurement procedure based on ID-MS are outlined.

Keywords: HbA2; standardization; traceability; β-thalassemia.

Publication types

  • Review