MiR-4282 inhibits proliferation, invasion and metastasis of human breast cancer by targeting Myc

Eur Rev Med Pharmacol Sci. 2018 Dec;22(24):8763-8771. doi: 10.26355/eurrev_201812_16643.

Abstract

Objective: To investigate the expression and role of MicroRNA 4282 (miR-4282) in the development of breast cancer.

Materials and methods: In situ hybridization was performed to investigate the expression of miR-4282 in human breast cancer tissues. Cell lines stably over-expressed miR-4282 were established by lentivirus transfection. The effects of miR-4282 on the biological behavior of breast cancer cells were then explored in vitro.

Results: The results showed that miR-4282 was down-regulated in human breast cancer tissues and was particularly in invasive and metastatic tumors. In addition, the expression of miR-4282 was related to the occurrence of metastasis and the clinical grade of breast cancer. Recovery of miR-4282 expression in the cell could inhibit the proliferation of breast cancer cells, blocked G1-S phase and promoted breast cancer cell apoptosis, inhibited breast cancer cell migration and invasion. Besides, miR-4282 also significantly enhanced the sensitivity of breast cancer cells to paclitaxel. The results of bioinformatics analysis combined with qRT-PCR and Western blot demonstrated that Myc might be the target gene of miR-4282 in breast cancer.

Conclusions: miR-4282 inhibited the occurrence and development of breast cancer by regulating Myc, which made it a new target for clinical diagnosis and treatment of breast cancer.

MeSH terms

  • Breast Neoplasms / genetics*
  • Breast Neoplasms / pathology
  • Cell Movement / genetics
  • Cell Proliferation / genetics
  • Down-Regulation
  • Female
  • Gene Expression Regulation, Neoplastic*
  • HEK293 Cells
  • Humans
  • MCF-7 Cells
  • MicroRNAs / metabolism*
  • Neoplasm Invasiveness / genetics
  • Neoplasm Invasiveness / pathology
  • Proto-Oncogene Proteins c-myc / genetics*

Substances

  • MIRN-4282 microRNA, human
  • MYC protein, human
  • MicroRNAs
  • Proto-Oncogene Proteins c-myc