DNA barcoding of fish fauna from low order streams of Tapajós River basin

Karen Larissa Auzier Guimarães; Marcos Paulo Alho de Sousa; Frank Raynner Vasconcelos Ribeiro; Jorge Ivan Rebelo Porto; Luís Reginaldo Ribeiro Rodrigues

doi:10.1371/journal.pone.0209430

DNA barcoding of fish fauna from low order streams of Tapajós River basin

PLoS One. 2018 Dec 21;13(12):e0209430. doi: 10.1371/journal.pone.0209430. eCollection 2018.

Authors

Karen Larissa Auzier Guimarães^{1

2}, Marcos Paulo Alho de Sousa^{1

2}, Frank Raynner Vasconcelos Ribeiro³, Jorge Ivan Rebelo Porto⁴, Luís Reginaldo Ribeiro Rodrigues^{1

2}

Affiliations

¹ Laboratório de Genética & Biodiversidade, Instituto de Ciências da Educação, Universidade Federal do Oeste do Pará, Santarém, Pará, Brazil.
² Programa de Pós Graduação em Recursos Naturais da Amazônia, Universidade Federal do Oeste do Pará, Santarém, Pará, Brazil.
³ Coleção Ictiológica, Instituto de Ciências e Tecnologia das Águas, Universidade Federal do Oeste do Pará, Santarém, Pará, Brazil.
⁴ Núcleo de Apoio a Pesquisa no Pará, Instituto Nacional de Pesquisas da Amazônia, Santarém, Pará, Brazil.

Abstract

The Amazon basin harbors a megadiverse fish fauna spread in an intricate network of big rivers and small streams. The Amazonian streams are home of many small sized fishes that remains poorly documented. In order to accelerate the scientific knowledge on these important aquatic systems we adopted a modern integrative approach joining morphology and molecular tools to investigate the ichthyofauna assemblages from low order streams situated on the lower Tapajós River Basin. Cytochrome c Oxidase I (COI) DNA barcodes from 252 specimens collected from 10 stream sites were obtained. The combined analysis revealed 29 species, 21 genera and 11 families. Cryptic diversity was evidenced in Knodus sp.1, Aequidens epae and Copella callolepis, in which deep genetic divergence were detected (intraspecific distances: 20.48%, 7.99% and 3.77%, respectively). The putative new species showed closer relationships with their counterparts occurring in the Tapajós-Xingu water drainages.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Biodiversity*
Brazil
Conservation of Natural Resources
DNA Barcoding, Taxonomic*
Electron Transport Complex IV / genetics
Fish Proteins / genetics
Fishes / genetics*
Phylogeny
Rivers

Substances

Fish Proteins
Electron Transport Complex IV

Grants and funding

MPAS received Mastership from Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES); KLAG received Scholarship PIBIC/CNPq/UFOPA. This research is part of the Project 09 associated to the Brazilian Barcoding of Life (BrBOL-PRJ09 to JIRP) granted by CNPq. Supplemental funds were provided from CAPES-PROAmazônia (AUXPE-3318/2013 to LRRR). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.