Immunization against streptococcus pneumoniae is for the prophylaxis of pneumococcus diseases in an increased number of risk patients and because of an increasing presence of antibiotica resistance in present days necessary. In order to get better possibilities to test the immunization effect, improved methods for detection of antibodies are applied. In this publication a double sandwich-EIA and a protein-coupled EIA are presented and compared. The double sandwich-EIA has proved to be superior with regard to its day by day variability of 12.5% and its series variability of 3.5% and to be of the same value compared with international developments. The production of most of antigens and antisera is described and the application of gamma-irradiated micro-plates is proposed.