Abstract
In A-to-I RNA editing, adenosine is converted to inosine in double-stranded regions of RNAs. Inosine, an abundant epitranscriptomic mark, contributes to a wide range of biological processes by regulating gene expression post-transcriptionally. To understand the effect of A-to-I RNA editing on regulation of the epitranscriptome, accurate mapping of inosines is necessary. To this end, we established a biochemical method called inosine chemical erasing sequencing (ICE-seq) that enables unbiased and reliable identification of A-to-I RNA editing sites throughout the transcriptome. Here, we describe our updated protocol for ICE-seq in the human transcriptome.
Keywords:
A-to-I RNA editing; High-throughput sequencing; ICE-seq.
Copyright © 2018 The Authors. Published by Elsevier Inc. All rights reserved.
Publication types
-
Research Support, Non-U.S. Gov't
MeSH terms
-
Adenosine / chemistry
-
Adenosine / metabolism
-
Animals
-
DNA, Complementary / chemistry
-
DNA, Complementary / genetics
-
DNA, Complementary / metabolism
-
Ethanol / chemistry
-
Ethylamines / chemistry
-
High-Throughput Nucleotide Sequencing / methods*
-
Humans
-
Inosine / chemistry
-
Inosine / metabolism
-
Molecular Sequence Annotation / methods*
-
Nitriles / chemistry
-
Poly A / chemistry
-
Poly A / genetics
-
Poly A / metabolism
-
RNA Editing*
-
RNA, Double-Stranded / chemistry
-
RNA, Double-Stranded / genetics
-
RNA, Double-Stranded / metabolism
-
RNA, Messenger / chemistry*
-
RNA, Messenger / genetics
-
RNA, Messenger / metabolism
-
Transcriptome*
Substances
-
DNA, Complementary
-
Ethylamines
-
Nitriles
-
RNA, Double-Stranded
-
RNA, Messenger
-
Poly A
-
Ethanol
-
Inosine
-
Adenosine
-
triethylamine