Synthesis of antithrombin III and alpha-1-antitrypsin by the perfused rat liver

Biochim Biophys Acta. 1978 Apr 3;539(4):496-504. doi: 10.1016/0304-4165(78)90083-1.


Livers isolated from control or turpentine-injected rats were perfused for 3 h with human red cells suspended in Krebs-Henseleit solution containing bovine serum albumin, dextran, glucose, heparin, cortisol, insulin, a mixture of 20 amino acids and [3H]leucine. Changes in the concentrations of antithrombin III and alpha-1-antitrypsin were evaluated by rocket immunoelectrophoresis using specific antisera, and incorporation of the 3H radioactivity into the total protein, albumin, antitrhombin III and alpha-1-antitrypsin in the perfusate was measured. The results indicate that both antithrombin III and alpha-1-antitrypsin are synthesized in the liver. Local inflammation induced in the liver donors moderately stimulated the synthesis of alpha-1-antitrypsin but it affected only marginally that of antithrombin III.

Publication types

  • Comparative Study

MeSH terms

  • Albumins / biosynthesis
  • Animals
  • Antithrombins / biosynthesis*
  • Leucine / metabolism
  • Liver / metabolism*
  • Male
  • Perfusion
  • Protein Biosynthesis
  • Rats
  • Turpentine / pharmacology
  • alpha 1-Antitrypsin / biosynthesis*


  • Albumins
  • Antithrombins
  • alpha 1-Antitrypsin
  • Leucine
  • Turpentine