Association between MEG3/miR-181b polymorphisms and risk of ischemic stroke

Lipids Health Dis. 2018 Dec 22;17(1):292. doi: 10.1186/s12944-018-0941-z.

Abstract

Background: Recent evidence suggests that long non-coding RNAs (lncRNAs) are key regulators in the pathological process of ischemic stroke (IS). Maternally expressed gene 3 (MEG3) was observed to be up-regulated in IS, acting as a competing endogenous RNA for miR-181b to regulate ischemic brain injury. The purpose of this study was to evaluate the association of tagSNPs in MEG3 (i.e., rs7158663 and rs4081134) and miR-181b rs322931 with IS risk.

Methods: Genomic DNA was extracted from blood samples of 509 patients with IS and 668 healthy controls. Genotyping of MEG3 rs7158663, rs4081134, and miR-181b rs322931 was performed by TaqMan assay. The transcriptional activity was measured using the Dual-Luciferase Reporter Assay kit.

Results: Single-site analysis revealed a significantly higher risk of IS being associated with miR-181b rs322931 CT and CT/TT genotypes (CT vs. CC: adjusted OR = 1.48, 95% CI: 1.13-1.95, P = 0.005; CT/TT vs. CC: adjusted OR = 1.52, 95% CI: 1.17-1.97, P = 0.002). Combined analyses revealed that combined genotypes (rs7158663 GG + rs322931 CT/TT and rs7158663 AG/AA + rs322931 CT/TT) increased IS risk compared to genotypes of rs7158663 GG + rs322931 CC. Stratification analyses showed that patients carrying miR-181b rs322931 CT/TT genotypes had higher levels of low-density lipoprotein cholesterol (LDL_C) (P = 0.01). Moreover, results from logistic regression analysis showed that rs322931 CT/TT genotypes were risk factors besides hypertension, total cholesterol, triglyceride, and LDL_C. Further dual-luciferase reporter assay showed that the rs322931 T allele had lower levels of luciferase activity than the rs322931 C allele.

Conclusion: These findings indicate that miR-181b rs322931 may singly or jointly contribute to the risk of IS.

Keywords: Ischemic stroke; Long non-coding RNAs; Maternally expressed gene 3; Polymorphism; miR-181.

MeSH terms

  • Aged
  • Asian Continental Ancestry Group
  • Brain Ischemia*
  • Female
  • Gene Expression Regulation
  • Genetic Predisposition to Disease*
  • Humans
  • Male
  • MicroRNAs / genetics*
  • Middle Aged
  • Polymorphism, Single Nucleotide*
  • RNA, Long Noncoding / genetics*
  • Stroke / genetics*
  • Stroke / metabolism

Substances

  • MEG3 non-coding RNA, human
  • MIrn181 microRNA, human
  • MicroRNAs
  • RNA, Long Noncoding