Antennal transcriptome analysis and expression profiles of odorant binding proteins in Clostera restitura

Abstract

Clostera restitura Walker (Lepidoptera: Notodontidae) is one of the most destructive defoliators of poplars in China. We constructed an antennal transcriptome using Illumina Hiseq 2500™ sequencing and characterized the expression profiles of odorant binding proteins for better understanding of the olfactory receptive system and the role of putative olfactory proteins in C. restitura. A total of 165 transcripts were identified, including 43 transcripts encoding putative odorant-binding proteins (OBPs), 13 chemosensory proteins (CSPs), 78 odorant receptors (ORs), 15 ionotropic receptors (IRs), 13 gustatory receptors (GRs), and 3 sensory neuron membrane proteins (SNMPs). Furthermore, we systematically analyzed expression patterns of eight OBPs from different tissues of both C. restitura sexes by using reverse transcription PCR and quantitative real time PCR (RT-qPCR). The expression level of CresGOBP2 in female antennae was approximately two times higher than in males, and two pheromone binding proteins PBPs (CresPBP1 and -PBP3) and three OBPs (CresOBP9, -10, and -16) were more highly enriched in male antennae than in female antennae. CresOBP10 showed a remarkably high expression in legs compared to other studied insects. Our results suggested that these proteins might play a key role in foraging, seeking mates, and host recognition in C. restitura. Our findings provided a foundation for future studies on the molecular mechanisms controlling the olfactory system in C. restitura and potential novel targets for pest control strategies.

Keywords: Chemosensory proteins; Clostera restitura; Expression patterns; Odorant-binding proteins; Phylogenetic analysis.