Pancreatic proglucagon processing: isolation and structures of glucagon and glucagon-like peptide from gene I

Endocrinology. 1988 Dec;123(6):2639-45. doi: 10.1210/endo-123-6-2639.

Abstract

The anglerfish endocrine pancreas expresses two different genes for preproglucagon. The regions of the two proglucagons that correspond to glucagon have different sequences, as do the two glucagon-like peptides (GLPs). The products derived from processing the more abundant proglucagon-II have recently been determined. However, it was not known whether proglucagon-I was processed to similar products. The two major biologically active products of preproglucagon-I processing (glucagon-I and GLP-I) have now been purified to homogeneity. Their structures were determined using automated gas phase Edman degradation, tryptic mapping, and fast atom bombardment mass spectrometry. The preproglucagon-I-processing sites were identified. Glucagon-I represents residues 53-81, and GLP-I corresponds to preproglucagon-I-(91-124) (numbering from the initiator Met).

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Chromatography, Gel
  • Chromatography, High Pressure Liquid
  • DNA / genetics
  • Fishes / metabolism*
  • Glucagon / genetics*
  • Glucagon / isolation & purification
  • Glucagon / metabolism
  • Glucagon-Like Peptides
  • Islets of Langerhans / metabolism*
  • Molecular Sequence Data
  • Peptide Fragments
  • Peptides / genetics*
  • Peptides / isolation & purification
  • Peptides / metabolism
  • Proglucagon
  • Protein Precursors / genetics*
  • Protein Precursors / metabolism
  • Protein Processing, Post-Translational*
  • Trypsin

Substances

  • Peptide Fragments
  • Peptides
  • Protein Precursors
  • Proglucagon
  • Glucagon-Like Peptides
  • glucagon-like-immunoreactivity
  • DNA
  • Glucagon
  • Trypsin