The Virus Pathogen Resource (ViPR; www.viprbrc.org ) is a US National Institute of Allergy and Infectious Diseases (NIAID)-sponsored Bioinformatics Resource Center providing bioinformatics support for major human viral pathogens. The hepatitis C virus (HCV) portal of ViPR facilitates basic research and development of diagnostics and therapeutics for HCV, by providing a comprehensive collection of HCV-related data integrated from various sources, a growing suite of analysis and visualization tools for data mining and hypothesis generation, and personal Workbench spaces for data storage and sharing. This chapter introduces the data and functionality provided by the ViPR HCV portal. It describes example workflows for (1) searching HCV genome and protein sequences, (2) conducting phylogenetic analysis, and (3) analyzing sequence variations using pattern search for amino acid substitutions in proteins, single nucleotide variation calculation, metadata-driven comparison, and sequence feature variant type analysis. All data and tools are freely available via the ViPR HCV portal at https://www.viprbrc.org/brc/home.spg?decorator=flavi_hcv .
Keywords: Annotation; Antiviral; Comparative genomics; Database; Drug resistance; Genotype; Mature peptides; Phylogenetics; ViPR; Virus Pathogen Resource.