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. 2018 Dec 20;45(12):651-662.
doi: 10.1016/j.jgg.2018.11.003. Epub 2018 Dec 9.

Loss of miR-83 Extends Lifespan and Affects Target Gene Expression in an Age-Dependent Manner in Caenorhabditis Elegans

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Loss of miR-83 Extends Lifespan and Affects Target Gene Expression in an Age-Dependent Manner in Caenorhabditis Elegans

Emmanuel Enoch Dzakah et al. J Genet Genomics. .

Abstract

MicroRNAs (miRNAs) are short non-coding RNAs that are involved in the post-transcriptional regulation of protein-coding genes. miRNAs modulate lifespan and the aging process in a variety of organisms. In this study, we identified a role of miR-83 in regulating lifespan of Caenorhabditis elegans. mir-83 mutants exhibited extended lifespan, and the overexpression of miR-83 was sufficient to decrease the prolonged lifespan of the mutants. We observed upregulation of the expression levels of a set of miR-83 target genes in young mir-83 mutant adults; while different sets of genes were upregulated in older mir-83 mutant adults. In vivo assays showed that miR-83 regulated expression of target genes including din-1, spp-9 and col-178, and we demonstrated that daf-16 and din-1 were required for the extension of lifespan in the mir-83 mutants. The regulation of din-1 by miR-83 during aging resulted in the differential expression of din-1 targets such as gst-4 and gst-10. In daf-2 mutants, the expression level of miR-83 was significantly reduced compared to wild-type animals. We identified a role for miR-83 in modulating lifespan in C. elegans and provided molecular insights into its functional mechanism.

Keywords: C. elegans; Longevity; daf-2; din-1; miR-83.

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