Correlation of Leukocyte Subtypes, Neutrohyl Lymphocyte Ratio, and Functional Outcome in Brain Metastasis

Kiking Ritarwan; Irina Keumala Nasution; Iswandi Erwin; Nerdy Nerdy

doi:10.3889/oamjms.2018.477

Correlation of Leukocyte Subtypes, Neutrohyl Lymphocyte Ratio, and Functional Outcome in Brain Metastasis

Open Access Maced J Med Sci. 2018 Dec 15;6(12):2333-2336. doi: 10.3889/oamjms.2018.477. eCollection 2018 Dec 20.

Authors

Kiking Ritarwan¹, Irina Keumala Nasution¹, Iswandi Erwin¹, Nerdy Nerdy²

Affiliations

¹ Department of Neurology, Faculty of Medicine, Universitas Sumatera Utara, Padang Bulan, Medan Baru, Medan, Sumatera Utara, Indonesia.
² Department of Pharmaceutical Chemistry, Academy of Pharmacy Yayasan Tenaga Pembangunan Arjuna, Pintubosi, Laguboti, Toba Samosir, Sumatera Utara, Indonesia.

Abstract

Background: As the most cause of death in patients with solid extracranial malignancy, brain metastasis (BM) nowadays being studied extensively especially on how to find a reliable laboratory marker that can correlate with its clinical outcome. Leukocyte subtypes, primarily neutrophils and lymphocytes and its ratio known as Neutrophils-Lymphocyte Ratio (NLR) have been known before its relationship with progressivity of BM from other solid tumours.

Aim: The objectives of this research to study the correlation of leukocyte subtypes, neutrophil-lymphocyte ratio & functional outcome in brain metastasis.

Methods: The study subjects were recruited consecutively from the study population. Venous blood was taken 5 ml of venous blood samples done in the first day of admission on emergency department and neurology clinic of Neurology Department of Adam Malik General Hospital before any drug injections. Samples were kept in vacutainer tubes containing ethylenediaminetetraacetic acid (EDTA) and sent to Department of Clinical Pathology laboratory of Adam Malik General Hospital, immediately centrifuged at 3100 rpm for 10 minutes in -20°C temperature and analysed using Sysmex XT-2000i. Functional outcome of the patient assessed using Karnofsky performance scale (KPS) in a cross-sectional manner with laboratory analysis.

Results: We conduct a mean differences and correlational leukocytes and its subsets analysis of 72 BM patients resulting on significant positive correlation on lymphocyte percentage (r = 0.383, p = 0.001) and lymphocyte absolute (r = 0.265, p = 0.024), also significant negative correlation on neutrophils (r = -0.240, p = 0.042) and NLR (r = -0.432, p < 0.001) with Karnofsky Performance Scale (KPS).

Conclusion: Increased lymphocyte absolute and lymphocyte percentage correlated significantly (p < 0.05) with better KPS, while elevated neutrophils percentage and increased NLR show significant correlation with worse outcome of BM patients.

Keywords: Brain Metastasis; Karnofsky Performance Scale; Neutrophils-Lymphocyte Ratio.