In vitro selection of DNA aptamers recognizing drug-resistant ovarian cancer by cell-SELEX

Junqing He; Junyan Wang; Nan Zhang; Luyao Shen; Linlin Wang; Xiao Xiao; Yan Wang; Tao Bing; Xiangjun Liu; Songqing Li; Dihua Shangguan

doi:10.1016/j.talanta.2018.10.028

In vitro selection of DNA aptamers recognizing drug-resistant ovarian cancer by cell-SELEX

Talanta. 2019 Mar 1:194:437-445. doi: 10.1016/j.talanta.2018.10.028. Epub 2018 Oct 12.

Authors

Junqing He¹, Junyan Wang², Nan Zhang³, Luyao Shen², Linlin Wang², Xiao Xiao², Yan Wang¹, Tao Bing², Xiangjun Liu², Songqing Li⁴, Dihua Shangguan⁵

Affiliations

¹ College of Chemistry, Xiangtan University, Xiangtan 411105, China; Department Beijing National Laboratory for Molecular Sciences, Key Laboratory of Analytical Chemistry for Living Biosystems, CAS Research/Education Center for Excellence in Molecular Sciences, Institute of Chemistry, Chinese Academy of Sciences, Beijing 100190, China.
² Department Beijing National Laboratory for Molecular Sciences, Key Laboratory of Analytical Chemistry for Living Biosystems, CAS Research/Education Center for Excellence in Molecular Sciences, Institute of Chemistry, Chinese Academy of Sciences, Beijing 100190, China; University of Chinese Academy of Sciences, Beijing 100049, China.
³ Department Beijing National Laboratory for Molecular Sciences, Key Laboratory of Analytical Chemistry for Living Biosystems, CAS Research/Education Center for Excellence in Molecular Sciences, Institute of Chemistry, Chinese Academy of Sciences, Beijing 100190, China; University of Chinese Academy of Sciences, Beijing 100049, China. Electronic address: hszhang@iccas.ac.cn.
⁴ College of Chemistry, Xiangtan University, Xiangtan 411105, China. Electronic address: lisq65@126.com.
⁵ Department Beijing National Laboratory for Molecular Sciences, Key Laboratory of Analytical Chemistry for Living Biosystems, CAS Research/Education Center for Excellence in Molecular Sciences, Institute of Chemistry, Chinese Academy of Sciences, Beijing 100190, China; University of Chinese Academy of Sciences, Beijing 100049, China. Electronic address: sgdh@iccas.ac.cn.

PMID: 30609555
DOI: 10.1016/j.talanta.2018.10.028

Abstract

Ovarian cancer is regarded as the most lethal gynecologic malignancy with poor prognosis and high mortality rate. Drug-resistance was thought to be the main obstacle to improving overall survival rate of ovarian cancer. New ligands for drug-resistant ovarian cancer cells have potential for the development of diagnosis and therapy of ovarian cancer. In present work, we reported two aptamers, HF3-58 and HA5-68 generated by cell-SELEX, against a paclitaxel-resistant ovarian cancer cell line (A2780T). Both two aptamers exhibited high selectivity and strong affinity to target cells with low nanomolar dissociation constants. The binding of aptamers to target cells was independent of divalent ions, and was tolerant of incubation temperature and nucleases in serum. Molecular targets of the two aptamers were preliminarily demonstrated to be two different glycoproteins on cell surface of A2780T cells. Owing to the structure stability and high resistance to nuclease, these two aptamers had good performance in the detection of drug-resistant ovarian cancer cells in human serum.

Keywords: Aptamer; Cell-SELEX; Drug resistance; Molecular probe; Ovarian cancer.

MeSH terms

Aptamers, Nucleotide / blood
Aptamers, Nucleotide / genetics
Aptamers, Nucleotide / metabolism*
Base Sequence
Cell Line, Tumor
Cell Membrane / metabolism
Drug Resistance, Neoplasm*
Female
Humans
Ovarian Neoplasms / metabolism
Ovarian Neoplasms / pathology*
SELEX Aptamer Technique*

Substances

Aptamers, Nucleotide