Fluorescent eggshell membrane-templated gold nanoclusters (Au-ESM) can be obtained in a facile and low-cost manner in this study. The fluorescence of the Au-ESM may be significantly quenched by mercapto-compounds and peroxidase-like activity of Au-ESM could be regulated by the reaction process with glutathione. Moreover, the catalytic activity of the mimetic enzyme membrane could be modulated by immunoreactions. An immunoassay for colorimetric determination of staphylococcal enterotoxins B (SE-B) using colored gold nanoparticles was established based on the catalytic activity adjusted by the target molecules. This colorimetric assay can detect SE-B at the concentration range of 0.4-20 ng/mL and the limit of detection (LOD) is 0.12 ng/mL. As a practical application, the proposed colorimetric assay was further utilized to detect SE-B in food samples such as flour, corn and rice, requiring very low volume of sample and exhibiting great sensitivity and high accuracy, which provides promising platform for development of point-of-care diagnostic devices with biomedical and food safety applications.
Keywords: Colorimetric detection; Egg shell membrane; Glutathione; Regulatable peroxidase-like activity; Staphylococcal enterotoxin B.
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