Establishment of an induced pluripotent stem cell line (FDEENTi001-A) from a patient with pathological myopia

Xinyue Bai; Xianjie Yang; Yun Cheng; Ling Chen

doi:10.1016/j.scr.2018.101369

Establishment of an induced pluripotent stem cell line (FDEENTi001-A) from a patient with pathological myopia

Stem Cell Res. 2019 Jan:34:101369. doi: 10.1016/j.scr.2018.101369. Epub 2018 Dec 14.

Authors

Xinyue Bai¹, Xianjie Yang², Yun Cheng¹, Ling Chen³

Affiliations

¹ Department of Ophthalmology & Vision Science, Eye & ENT Hospital, Shanghai Medical School, Fudan University, Shanghai, China; Key NHC Key Laboratory of Myopia (Fudan University), Laboratory of Myopia, Chinese Academy of Medical Sciences, China.
² Jules Stein Eye Institute, University of California Los Angeles, California, USA.
³ Department of Ophthalmology & Vision Science, Eye & ENT Hospital, Shanghai Medical School, Fudan University, Shanghai, China; Key NHC Key Laboratory of Myopia (Fudan University), Laboratory of Myopia, Chinese Academy of Medical Sciences, China. Electronic address: linglingchen98@hotmail.com.

Abstract

Pathological myopia (PM) is a retinal degenerative disease with an increasing prevalence in Asia. The peripheral blood mononuclear cells (PBMCs) from a patient with PM were successfully reprogrammed to induced pluripotent stem cells (iPSCs) using integration-free method, Sendai viral (SeV) vectors expressing OCT4, SOX2, KLF4 and C-MYC. This line may provide a useful resource for exploring the pathogenesis of PM.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Cell Culture Techniques / methods*
Cell Line
Humans
Induced Pluripotent Stem Cells / pathology*
Kruppel-Like Factor 4
Male
Mice
Middle Aged
Myopia, Degenerative / pathology*

Abstract

Publication types

MeSH terms

Grants and funding