Enterobacter huaxiensis sp. nov. and Enterobacter chuandaensis sp. nov., recovered from human blood

Int J Syst Evol Microbiol. 2019 Mar;69(3):708-714. doi: 10.1099/ijsem.0.003207.


Enterobacter strains 090008T and 090028T were recovered from the blood of two different patients at West China Hospital, Chengdu, PR China in 2017. Phylogenetic analysis based on the 16S rRNA gene and multi-locus sequence analysis of the rpoB, gyrB, infB and atpD housekeeping genes revealed that the two strains were distinct from any previously known species of the genus Enterobacter. Both average nucleotide identity (ANI) and in silico DNA-DNA hybridization (isDDH) values between strains 090008T, 090028T and type strains of all known Enterobacter species were lower than the cut-off (≥95-96 % for ANI and ≥70 % for isDDH) to define a bacterial species. The major cellular fatty acids of strains 090008T and 090028T are C16 : 0, C17 : 0cyclo and C18 : 1ω7c, which are similar to other Enterobacter species, and the genomic DNA G+C content was 55.73 and 55.68 mol%, respectively. Strain 090008T can be differentiated from other Enterobacter species by its ability to ferment sucrose, melibiose and d-arabitol, but with a negative methyl α-d-mannopyranoside reaction. Strain 090028T can ferment potassium gluconate, but is negative for l-fucose, mdecarboxylase, deaminase aelibiose and d-sorbitol, which distinguishes it from all other Enterobacter species. Genotypic and phenotypic characteristics indicate that strains 090008T and 090028T represent two novel species of the genus Enterobacter, for which the names Enterobacter huaxiensissp. nov. and Enterobacter chuandaensis sp. nov. are proposed, respectively. The type strain of Enterobacter huaxiensissp. nov. is 090008T (=GDMCC1.1426T=CCTCC AB 2018174T=CNCTC 7648T) and the type strain of Enterobacter chuandaensissp. nov. is 090028T (=GDMCC1.1427T=CCTCC AB 2018173T=CNCTC 7649T).

Keywords: Enterobacter; blood; genome.

MeSH terms

  • Bacterial Typing Techniques
  • Base Composition
  • Blood / microbiology*
  • China
  • DNA, Bacterial / genetics
  • Enterobacter / classification*
  • Enterobacter / isolation & purification
  • Fatty Acids / chemistry
  • Genes, Bacterial
  • Humans
  • Nucleic Acid Hybridization
  • Phylogeny*
  • RNA, Ribosomal, 16S / genetics
  • Sequence Analysis, DNA


  • DNA, Bacterial
  • Fatty Acids
  • RNA, Ribosomal, 16S