Label-free real-time ultrasensitive monitoring of non-small cell lung cancer cell interaction with drugs

Hailang Dai; Yihang Jiao; Zhangchi Sun; Zhuangqi Cao; Xianfeng Chen

doi:10.1364/BOE.9.004149

Label-free real-time ultrasensitive monitoring of non-small cell lung cancer cell interaction with drugs

Biomed Opt Express. 2018 Aug 7;9(9):4149-4161. doi: 10.1364/BOE.9.004149. eCollection 2018 Sep 1.

Authors

Hailang Dai^{1

2}, Yihang Jiao^{1

2}, Zhangchi Sun³, Zhuangqi Cao¹, Xianfeng Chen^{1

2}

Affiliations

¹ The State Key Laboratory on Fiber Optic Local Area Communication Networks and Advanced Optical Communication Systems, school of Physics and Astronomy, Shanghai JiaoTong University, Shanghai 200240, China.
² Collaborative Innovation Center of IFSA (CICIFSA), Shanghai Jiao Tong University, Shanghai 200240, China.
³ Zhejiang Rongjun Hospital, Jiaxing 314000, China.

Abstract

The timely discovery of cancer cell resistance in clinical processing and the accurate calculation of drug dosage to reduce and inhibit tumour growth factor in cancer patients are promising technologies in cancer therapy. Here, an optofluidic resonator effectively detects drug interactions with cancer cell processing in real time and enables the calculation of label-free drug-non-small cell lung cancer (NSCLC) epidermal growth factor receptor (EGFR) and binding ratios using molecular fluorescence intensity. According to clinical test and in vivo experimental data, the efficiencies of gefitinib and erlotinib are only 37% and 12% compared to AZD9291, and 0.300 μg of EGFR inactivation requires 0.484 μg of AZD9291, 0.815 μg of gefitinib and 1.348 μg of erlotinib. Experimental results show that the present method allows for the performance detection of drug resistance and for the evaluation of dosage usage.

Keywords: (140.0140) Lasers and laser optics; (140.4780) Optical resonators; (170.0170) Medical optics and biotechnology; (170.4580) Optical diagnostics for medicine; (170.6930) Tissue.