Multivitamin and Mineral Supplementation Containing Phytonutrients Scavenges Reactive Oxygen Species in Healthy Subjects: A Randomized, Double-Blinded, Placebo-Controlled Trial

Nutrients. 2019 Jan 5;11(1):101. doi: 10.3390/nu11010101.


Phytonutrients and vitamin and mineral supplementation have been reported to provide increased antioxidant capacity in humans; however, there is still controversy. In the current clinical trial, we examined the antioxidant and DNA protection capacity of a plant-based, multi-vitamin/mineral, and phytonutrient (PMP) supplementation in healthy adults who were habitually low in the consumption of fruits and vegetables. This study was an eight-week, double-blind, randomized, parallel-arm, and placebo-controlled trial. PMP supplementation for eight weeks reduced reactive oxygen species (ROS) and prevented DNA damage without altering endogenous antioxidant system. Plasma vitamins and phytonutrients were significantly correlated with ROS scavenging and DNA damage. In addition, gene expression analysis in PBMC showed subtle changes in superoxide metabolic processes. In this study, we showed that supplementation with a PMP significantly improved ROS scavenging activity and prevented DNA damage. However, additional research is still needed to further identify mechanisms of actions and the role of circulating phytonutrient metabolites.

Keywords: DNA damage; ROS scavenging; antioxidant capacity; human clinical study; phytonutrients.

Publication types

  • Randomized Controlled Trial

MeSH terms

  • Adult
  • Aged
  • Antioxidants / administration & dosage*
  • Antioxidants / analysis
  • DNA Damage / drug effects
  • Diet
  • Dietary Supplements
  • Double-Blind Method
  • Female
  • Free Radical Scavengers / administration & dosage*
  • Free Radical Scavengers / chemistry
  • Fruit
  • Humans
  • Male
  • Middle Aged
  • Minerals / administration & dosage*
  • Minerals / blood
  • Phytochemicals / administration & dosage*
  • Phytochemicals / blood
  • Placebos
  • Reactive Oxygen Species / blood*
  • Reactive Oxygen Species / chemistry
  • Vegetables
  • Vitamins / administration & dosage*
  • Vitamins / blood


  • Antioxidants
  • Free Radical Scavengers
  • Minerals
  • Phytochemicals
  • Placebos
  • Reactive Oxygen Species
  • Vitamins