Myogenin promoter-associated lncRNA Myoparr is essential for myogenic differentiation

EMBO Rep. 2019 Mar;20(3):e47468. doi: 10.15252/embr.201847468. Epub 2019 Jan 8.


Promoter-associated long non-coding RNAs (lncRNAs) regulate the expression of adjacent genes; however, precise roles of these lncRNAs in skeletal muscle remain largely unknown. Here, we characterize a promoter-associated lncRNA, Myoparr, in myogenic differentiation and muscle disorders. Myoparr is expressed from the promoter region of the mouse and human myogenin gene, one of the key myogenic transcription factors. We show that Myoparr is essential both for the specification of myoblasts by activating neighboring myogenin expression and for myoblast cell cycle withdrawal by activating myogenic microRNA expression. Mechanistically, Myoparr interacts with Ddx17, a transcriptional coactivator of MyoD, and regulates the association between Ddx17 and the histone acetyltransferase PCAF Myoparr also promotes skeletal muscle atrophy caused by denervation, and knockdown of Myoparr rescues muscle wasting in mice. Our findings demonstrate that Myoparr is a novel key regulator of muscle development and suggest that Myoparr is a potential therapeutic target for neurogenic atrophy in humans.

Keywords: DEAD box protein; chromatin; myogenesis; transcriptional regulation.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cell Cycle
  • Cell Differentiation / genetics*
  • Cell Line
  • DEAD-box RNA Helicases / metabolism
  • Gene Expression Regulation, Developmental
  • Humans
  • Mice, Inbred C57BL
  • Models, Biological
  • Muscle Development / genetics*
  • Muscle, Skeletal / metabolism
  • Muscular Atrophy / genetics
  • Muscular Atrophy / pathology
  • MyoD Protein / metabolism
  • Myoblasts / cytology
  • Myoblasts / metabolism
  • Myogenin / genetics*
  • Promoter Regions, Genetic*
  • Protein Binding
  • RNA, Long Noncoding / genetics
  • RNA, Long Noncoding / metabolism*
  • Transforming Growth Factor beta / metabolism
  • p300-CBP Transcription Factors / metabolism


  • MyoD Protein
  • Myogenin
  • RNA, Long Noncoding
  • Transforming Growth Factor beta
  • p300-CBP Transcription Factors
  • p300-CBP-associated factor
  • DEAD-box RNA Helicases