Nucleotide Sequence of the Ugp Genes of Escherichia Coli K-12: Homology to the Maltose System

Mol Microbiol. 1988 Nov;2(6):767-75. doi: 10.1111/j.1365-2958.1988.tb00088.x.

Abstract

The nucleotide sequence of the ugp genes of Escherichia coli K-12, which encode a phosphate-limitation inducible uptake system for sn-glycerol-3-phosphate and glycerophosphoryl diesters, was determined. The genetic organization of the operon differed from previously published results. A single promoter, containing a putative pho box, was detected by S1-nuclease mapping. The promoter is followed by four open reading frames, designated ugpB, A, E and C, which encode a periplasmic binding protein, two hydrophobic membrane proteins and a protein that is likely to couple energy to the transport system, respectively. The sequences of the proteins contain the characteristics of several other binding protein-dependent transport systems, but they seem to be particularly closely related to the maltose system.

Publication types

  • Comparative Study

MeSH terms

  • Amino Acid Sequence
  • Base Sequence
  • Biological Transport, Active
  • DNA, Bacterial / genetics
  • Escherichia coli / genetics*
  • Escherichia coli / metabolism
  • Genes, Bacterial*
  • Glycerophosphates / pharmacokinetics
  • Maltose / pharmacokinetics
  • Molecular Sequence Data
  • Phosphates / metabolism
  • Promoter Regions, Genetic
  • Restriction Mapping
  • Sequence Homology, Nucleic Acid

Substances

  • DNA, Bacterial
  • Glycerophosphates
  • Phosphates
  • Maltose
  • alpha-glycerophosphoric acid

Associated data

  • GENBANK/UNKNOWN