S1P mediates human amniotic cells proliferation induced by a 50-Hz magnetic field exposure via ERK1/2 signaling pathway

J Cell Physiol. 2019 Jun;234(6):7734-7741. doi: 10.1002/jcp.28102. Epub 2019 Jan 9.


Extremely low frequency electromagnetic field (ELF-EMF) is a kind of physical stimulus in public and occupational environment. Numerous studies have indicated that exposure of cells to ELF-EMF could promote cell proliferation. But the detailed mechanisms implicated in these proliferative processes remain unclear. In the present experiment, the possible roles of sphingosine-1-phosphate (S1P) in 50-Hz magnetic field (MF)-induced cell proliferation were investigated. Results showed that exposure of human amniotic (FL) cells to a 50-Hz MF with an intensity of 0.4 mT significantly enhanced ceramide metabolism, increased S1P production, activated extracellular signal regulated kinase 1/2 (ERK1/2), and promoted cell proliferation. All of these effects induced by MF exposure could be inhibited by SKI II, an inhibitor of sphingosine kinase (SphK). In addition, both the cell proliferative response and the ERK1/2 activation induced by MF exposure were blocked completely by U0126, a specific inhibitor of MEK (ERK kinases 1 and 2). Taken together, the findings in present study suggested that S1P mediated 50-Hz MF-induced cell proliferation via triggering ERK1/2 signal pathway.

Keywords: 50-Hz magnetic field (MF) exposure; extracellular signal regulated kinase (ERK); proliferation; sphingosine-1-phosphate (S1P).

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cell Line, Tumor
  • Cell Proliferation / physiology
  • Electromagnetic Fields / adverse effects
  • Humans
  • Lysophospholipids / metabolism*
  • MAP Kinase Signaling System / physiology*
  • Magnetic Fields / adverse effects*
  • Mitogen-Activated Protein Kinase 3 / metabolism
  • Phosphorylation
  • Phosphotransferases (Alcohol Group Acceptor) / metabolism
  • Sphingosine / analogs & derivatives*
  • Sphingosine / metabolism


  • Lysophospholipids
  • sphingosine 1-phosphate
  • Phosphotransferases (Alcohol Group Acceptor)
  • sphingosine kinase
  • MAPK3 protein, human
  • Mitogen-Activated Protein Kinase 3
  • Sphingosine