Lactobacillus salitolerans sp. nov., a novel lactic acid bacterium isolated from spent mushroom substrates

Int J Syst Evol Microbiol. 2019 Apr;69(4):964-969. doi: 10.1099/ijsem.0.003224. Epub 2019 Jan 10.


A taxonomic study of a Gram-stain-positive, rod-shaped, non-motile, non-spore-forming, catalase-negative bacterium, strain YK43T, isolated from spent mushroom substrates stored in Nagano, Japan was performed. Growth was detected at 15-45 °C, pH 5.0-8.5, and 0-10 % (w/v) NaCl. The genomic DNA G+C content of strain YK43T was 43.6 mol%. The predominant fatty acids were C16 : 0, C18 : 1 ω9c and summed feature 8. Based on 16S rRNA gene sequence analysis, the type strains of Lactobacillus acidipiscis (sequence similarity, 97.6 %) and Lactobacillus pobuzihii (97.4 %) were most closely related to YK43T. The average nucleotide identities were 74.1 % between strain YK43T and L. acidipiscis DSM 15836T and 74.0 % between YK43T and L. pobuzihii E100301T. Based on a multilocus sequence analysis, comparative genomic analysis and a range of phenotypic and chemotaxonomic characteristics, strain YK43T represents a novel species of the genus Lactobacillus, for which the name Lactobacillussalitolerans sp. nov. is proposed. The type strain is YK43T (=JCM 31331T = DSM 103433T).

Keywords: Lactobacillus salitolerans sp. nov.; spent mushroom substrates.

MeSH terms

  • Agaricales*
  • Bacterial Typing Techniques
  • Base Composition
  • DNA, Bacterial / genetics
  • Fatty Acids / chemistry
  • Japan
  • Lactobacillus / classification*
  • Lactobacillus / isolation & purification
  • Multilocus Sequence Typing
  • Phylogeny*
  • RNA, Ribosomal, 16S / genetics
  • Sequence Analysis, DNA


  • DNA, Bacterial
  • Fatty Acids
  • RNA, Ribosomal, 16S