Genome-wide analysis of ETV1 targets: Insights into the role of ETV1 in tumor progression

J Cell Biochem. 2019 Jun;120(6):8983-8991. doi: 10.1002/jcb.28169. Epub 2019 Jan 10.


ETS variant 1 (ETV1) is a key player in metastatic progression in several types of human cancers, yet the direct target genes of ETV1 and the mechanisms by which ETV1 exerts its deleterious function remain largely elusive. Here, we performed large-scale mapping and analysis of target loci of ETV1 in the prostate cancer cells LNCaP using the DNA adenine methyltransferase identification technique, we identified close to 800 direct targets for ETV1. Expression analysis using quantitative reverse transcription polymerase chain reaction confirmed a positive regulation by ETV1 in most of the genes examined. Furthermore, gene and pathway analysis unraveled new signaling pathways and biological networks that interact with ETV1. Our findings cast light on genes and networks regulated by ETV1, it also opens new fronts for studying the role of ETV1 and its target genes in tumorigenesis.

Keywords: DamID; ETS; ETV1; MMP; cancer.

MeSH terms

  • Cell Line, Tumor
  • Chromatin Immunoprecipitation / methods
  • DNA-Binding Proteins / metabolism*
  • Exome Sequencing
  • Gene Expression Profiling / methods*
  • Gene Expression Regulation, Neoplastic
  • Gene Regulatory Networks*
  • HEK293 Cells
  • Humans
  • Male
  • Prostatic Neoplasms / genetics*
  • Prostatic Neoplasms / metabolism
  • Protein Binding
  • Transcription Factors / metabolism*


  • DNA-Binding Proteins
  • ETV1 protein, human
  • Transcription Factors