Genomic analysis of three Clostridioides difficile isolates from urban water sources

Anaerobe. 2019 Apr:56:22-26. doi: 10.1016/j.anaerobe.2019.01.002. Epub 2019 Jan 8.


We investigated inflow of a wastewater treatment plant and sediment of an urban lake for the presence of Clostridioides difficile by cultivation and PCR. Among seven colonies we sequenced the complete genomes of three: two non-toxigenic isolates from wastewater and one toxigenic isolate from the urban lake. For all obtained isolates, a close genomic relationship with human-derived isolates was observed.

Keywords: Clostridioides difficile; Genomes; Sediment; Wastewater.

MeSH terms

  • Bacteriological Techniques
  • Berlin
  • Clostridioides difficile / classification
  • Clostridioides difficile / genetics*
  • Clostridioides difficile / isolation & purification*
  • Genome, Bacterial*
  • Genomics
  • Genotype
  • Phylogeny
  • Polymerase Chain Reaction
  • Sequence Analysis, DNA
  • Water Microbiology*