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. 2018 Nov 23;7(1):1535750.
doi: 10.1080/20013078.2018.1535750. eCollection 2018.

Minimal Information for Studies of Extracellular Vesicles 2018 (MISEV2018): A Position Statement of the International Society for Extracellular Vesicles and Update of the MISEV2014 Guidelines

Clotilde Théry  1 Kenneth W Witwer  2   3 Elena Aikawa  4   5 Maria Jose Alcaraz  6 Johnathon D Anderson  7 Ramaroson Andriantsitohaina  8 Anna Antoniou  9   10 Tanina Arab  11 Fabienne Archer  12 Georgia K Atkin-Smith  13 D Craig Ayre  14   15 Jean-Marie Bach  16 Daniel Bachurski  17 Hossein Baharvand  18   19 Leonora Balaj  20 Shawn Baldacchino  21 Natalie N Bauer  22 Amy A Baxter  13 Mary Bebawy  23 Carla Beckham  24 Apolonija Bedina Zavec  25 Abderrahim Benmoussa  26 Anna C Berardi  27 Paolo Bergese  28   29   30 Ewa Bielska  31 Cherie Blenkiron  32 Sylwia Bobis-Wozowicz  33 Eric Boilard  26 Wilfrid Boireau  34 Antonella Bongiovanni  35 Francesc E Borràs  36   37   38 Steffi Bosch  16 Chantal M Boulanger  39   40 Xandra Breakefield  41 Andrew M Breglio  42   43 Meadhbh Á Brennan  44   45   46 David R Brigstock  47   48 Alain Brisson  49 Marike Ld Broekman  50   51   52 Jacqueline F Bromberg  53   54 Paulina Bryl-Górecka  55 Shilpa Buch  56 Amy H Buck  57 Dylan Burger  58   59   60 Sara Busatto  61   30 Dominik Buschmann  62 Benedetta Bussolati  63 Edit I Buzás  64   65 James Bryan Byrd  66 Giovanni Camussi  67 David Rf Carter  68 Sarah Caruso  13 Lawrence W Chamley  69 Yu-Ting Chang  70 Chihchen Chen  71   72 Shuai Chen  73 Lesley Cheng  13 Andrew R Chin  74 Aled Clayton  75 Stefano P Clerici  76 Alex Cocks  75 Emanuele Cocucci  77   78 Robert J Coffey  79 Anabela Cordeiro-da-Silva  80 Yvonne Couch  81 Frank Aw Coumans  82 Beth Coyle  83 Rossella Crescitelli  84 Miria Ferreira Criado  85 Crislyn D'Souza-Schorey  86 Saumya Das  87 Amrita Datta Chaudhuri  3 Paola de Candia  88 Eliezer F De Santana  89 Olivier De Wever  90   91 Hernando A Del Portillo  92   93   94 Tanguy Demaret  95 Sarah Deville  96   97 Andrew Devitt  98 Bert Dhondt  90   99   91 Dolores Di Vizio  74 Lothar C Dieterich  100 Vincenza Dolo  101 Ana Paula Dominguez Rubio  102 Massimo Dominici  103   104 Mauricio R Dourado  105   106 Tom Ap Driedonks  107 Filipe V Duarte  108 Heather M Duncan  109   110 Ramon M Eichenberger  111 Karin Ekström  112 Samir El Andaloussi  113   114 Celine Elie-Caille  34 Uta Erdbrügger  115 Juan M Falcón-Pérez  116   117 Farah Fatima  118 Jason E Fish  119   120 Miguel Flores-Bellver  121 András Försönits  65 Annie Frelet-Barrand  34 Fabia Fricke  122   123 Gregor Fuhrmann  124   125   126 Susanne Gabrielsson  127 Ana Gámez-Valero  36   128 Chris Gardiner  129 Kathrin Gärtner  130 Raphael Gaudin  131   132 Yong Song Gho  133 Bernd Giebel  134 Caroline Gilbert  26 Mario Gimona  135 Ilaria Giusti  101 Deborah Ci Goberdhan  136 André Görgens  113   137   134 Sharon M Gorski  138   139 David W Greening  13 Julia Christina Gross  140   141 Alice Gualerzi  142 Gopal N Gupta  143 Dakota Gustafson  120 Aase Handberg  144   145 Reka A Haraszti  146 Paul Harrison  147 Hargita Hegyesi  65 An Hendrix  90   91 Andrew F Hill  13 Fred H Hochberg  148   149 Karl F Hoffmann  150 Beth Holder  151   152 Harry Holthofer  153 Baharak Hosseinkhani  154 Guoku Hu  56 Yiyao Huang  155   2 Veronica Huber  156 Stuart Hunt  157 Ahmed Gamal-Eldin Ibrahim  158 Tsuneya Ikezu  159 Jameel M Inal  160 Mustafa Isin  161 Alena Ivanova  162 Hannah K Jackson  83 Soren Jacobsen  163   164 Steven M Jay  165 Muthuvel Jayachandran  166 Guido Jenster  167 Lanzhou Jiang  13 Suzanne M Johnson  168 Jennifer C Jones  169 Ambrose Jong  170   171 Tijana Jovanovic-Talisman  172 Stephanie Jung  173 Raghu Kalluri  174 Shin-Ichi Kano  175 Sukhbir Kaur  176 Yumi Kawamura  177   178 Evan T Keller  179   180 Delaram Khamari  65 Elena Khomyakova  181   182 Anastasia Khvorova  146 Peter Kierulf  183 Kwang Pyo Kim  184 Thomas Kislinger  185   186 Mikael Klingeborn  187 David J Klinke 2nd  188   189 Miroslaw Kornek  190   191 Maja M Kosanović  192 Árpád Ferenc Kovács  65 Eva-Maria Krämer-Albers  193 Susanne Krasemann  194 Mirja Krause  195 Igor V Kurochkin  196 Gina D Kusuma  195   197 Sören Kuypers  198 Saara Laitinen  199 Scott M Langevin  200   201 Lucia R Languino  202 Joanne Lannigan  203 Cecilia Lässer  84 Louise C Laurent  204 Gregory Lavieu  1 Elisa Lázaro-Ibáñez  205 Soazig Le Lay  8 Myung-Shin Lee  206 Yi Xin Fiona Lee  207 Debora S Lemos  208 Metka Lenassi  209 Aleksandra Leszczynska  210 Isaac Ts Li  211 Ke Liao  56 Sten F Libregts  212 Erzsebet Ligeti  213 Rebecca Lim  195   197 Sai Kiang Lim  214 Aija Linē  215 Karen Linnemannstöns  140   141 Alicia Llorente  216 Catherine A Lombard  95 Magdalena J Lorenowicz  217 Ákos M Lörincz  213 Jan Lötvall  84 Jason Lovett  218 Michelle C Lowry  219 Xavier Loyer  39   40 Quan Lu  220 Barbara Lukomska  221 Taral R Lunavat  222 Sybren Ln Maas  223   224 Harmeet Malhi  225 Antonio Marcilla  226   227 Jacopo Mariani  228 Javier Mariscal  74 Elena S Martens-Uzunova  167 Lorena Martin-Jaular  1 M Carmen Martinez  8 Vilma Regina Martins  229 Mathilde Mathieu  1 Suresh Mathivanan  13 Marco Maugeri  230 Lynda K McGinnis  231 Mark J McVey  232   233 David G Meckes Jr  234 Katie L Meehan  235 Inge Mertens  236   97 Valentina R Minciacchi  237 Andreas Möller  238 Malene Møller Jørgensen  239   240 Aizea Morales-Kastresana  169 Jess Morhayim  241 François Mullier  242   243 Maurizio Muraca  244 Luca Musante  115 Veronika Mussack  62 Dillon C Muth  2 Kathryn H Myburgh  218 Tanbir Najrana  245 Muhammad Nawaz  230 Irina Nazarenko  246   247 Peter Nejsum  248 Christian Neri  249 Tommaso Neri  250 Rienk Nieuwland  82 Leonardo Nimrichter  251 John P Nolan  148 Esther Nm Nolte-'t Hoen  107 Nicole Noren Hooten  252 Lorraine O'Driscoll  219 Tina O'Grady  253 Ana O'Loghlen  254 Takahiro Ochiya  255 Martin Olivier  256 Alberto Ortiz  257   258   259 Luis A Ortiz  260 Xabier Osteikoetxea  261 Ole Østergaard  262   263 Matias Ostrowski  264 Jaesung Park  133 D Michiel Pegtel  265 Hector Peinado  266 Francesca Perut  267 Michael W Pfaffl  62 Donald G Phinney  268 Bartijn Ch Pieters  269 Ryan C Pink  68 David S Pisetsky  270   271 Elke Pogge von Strandmann  272 Iva Polakovicova  273   274 Ivan Kh Poon  13 Bonita H Powell  2 Ilaria Prada  275 Lynn Pulliam  276   277 Peter Quesenberry  278 Annalisa Radeghieri  28   30 Robert L Raffai  279   276 Stefania Raimondo  280 Janusz Rak  281   256 Marcel I Ramirez  282   283 Graça Raposo  284 Morsi S Rayyan  285 Neta Regev-Rudzki  286 Franz L Ricklefs  287 Paul D Robbins  288 David D Roberts  176 Silvia C Rodrigues  289   108 Eva Rohde  290   135   291 Sophie Rome  292 Kasper Ma Rouschop  293 Aurelia Rughetti  294 Ashley E Russell  295 Paula Saá  296 Susmita Sahoo  297 Edison Salas-Huenuleo  298   299 Catherine Sánchez  300 Julie A Saugstad  301 Meike J Saul  302 Raymond M Schiffelers  303 Raphael Schneider  120   304 Tine Hiorth Schøyen  2 Aaron Scott  305 Eriomina Shahaj  156 Shivani Sharma  306   307   308 Olga Shatnyeva  205 Faezeh Shekari  18 Ganesh Vilas Shelke  309   84 Ashok K Shetty  310   311 Kiyotaka Shiba  312 Pia R-M Siljander  313   314 Andreia M Silva  315   316   317 Agata Skowronek  318 Orman L Snyder 2nd  319 Rodrigo Pedro Soares  320 Barbara W Sódar  65 Carolina Soekmadji  238   321 Javier Sotillo  111 Philip D Stahl  322 Willem Stoorvogel  107 Shannon L Stott  323   324 Erwin F Strasser  325 Simon Swift  326 Hidetoshi Tahara  327 Muneesh Tewari  179   328   329 Kate Timms  330 Swasti Tiwari  331   332 Rochelle Tixeira  13 Mercedes Tkach  1 Wei Seong Toh  333 Richard Tomasini  334 Ana Claudia Torrecilhas  335 Juan Pablo Tosar  336   337 Vasilis Toxavidis  338 Lorena Urbanelli  339 Pieter Vader  303 Bas Wm van Balkom  340 Susanne G van der Grein  107 Jan Van Deun  90   91 Martijn Jc van Herwijnen  107 Kendall Van Keuren-Jensen  341 Guillaume van Niel  342 Martin E van Royen  343 Andre J van Wijnen  344 M Helena Vasconcelos  345   346   316 Ivan J Vechetti Jr  347 Tiago D Veit  348 Laura J Vella  349   350 Émilie Velot  351 Frederik J Verweij  342 Beate Vestad  352   353   354 Jose L Viñas  58   59   60 Tamás Visnovitz  65 Krisztina V Vukman  65 Jessica Wahlgren  355 Dionysios C Watson  356   357 Marca Hm Wauben  107 Alissa Weaver  358 Jason P Webber  75 Viktoria Weber  359 Ann M Wehman  360 Daniel J Weiss  361 Joshua A Welsh  169 Sebastian Wendt  362 Asa M Wheelock  363 Zoltán Wiener  65 Leonie Witte  140   141 Joy Wolfram  364   365   366 Angeliki Xagorari  367 Patricia Xander  368 Jing Xu  138   139 Xiaomei Yan  369 María Yáñez-Mó  370   371 Hang Yin  372 Yuana Yuana  373 Valentina Zappulli  374 Jana Zarubova  375   376   377 Vytautas Žėkas  378 Jian-Ye Zhang  379 Zezhou Zhao  2 Lei Zheng  155 Alexander R Zheutlin  285 Antje M Zickler  380 Pascale Zimmermann  381   382 Angela M Zivkovic  383 Davide Zocco  384 Ewa K Zuba-Surma  33
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Free PMC article

Minimal Information for Studies of Extracellular Vesicles 2018 (MISEV2018): A Position Statement of the International Society for Extracellular Vesicles and Update of the MISEV2014 Guidelines

Clotilde Théry et al. J Extracell Vesicles. .
Free PMC article

Abstract

The last decade has seen a sharp increase in the number of scientific publications describing physiological and pathological functions of extracellular vesicles (EVs), a collective term covering various subtypes of cell-released, membranous structures, called exosomes, microvesicles, microparticles, ectosomes, oncosomes, apoptotic bodies, and many other names. However, specific issues arise when working with these entities, whose size and amount often make them difficult to obtain as relatively pure preparations, and to characterize properly. The International Society for Extracellular Vesicles (ISEV) proposed Minimal Information for Studies of Extracellular Vesicles ("MISEV") guidelines for the field in 2014. We now update these "MISEV2014" guidelines based on evolution of the collective knowledge in the last four years. An important point to consider is that ascribing a specific function to EVs in general, or to subtypes of EVs, requires reporting of specific information beyond mere description of function in a crude, potentially contaminated, and heterogeneous preparation. For example, claims that exosomes are endowed with exquisite and specific activities remain difficult to support experimentally, given our still limited knowledge of their specific molecular machineries of biogenesis and release, as compared with other biophysically similar EVs. The MISEV2018 guidelines include tables and outlines of suggested protocols and steps to follow to document specific EV-associated functional activities. Finally, a checklist is provided with summaries of key points.

Keywords: ectosomes; exosomes; extracellular vesicles; guidelines; microparticles; microvesicles; minimal information requirements; reproducibility; rigor; standardization.

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References

    1. References, especially those provided to illustrate methods and approaches, are representative only, and are not meant to be a comprehensive review of the literature. Most references were derived from suggestions provided in the MISEV2018 Survey results. Each reference was checked by multiple authors. Citation implies deemed relevance of scientific content and not an endorsement by the authors or ISEV of any particular journal or editorial practice.
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