Statistical Positioning of Nucleosomes by Specific Protein-Binding to an Upstream Activating Sequence in Yeast

J Mol Biol. 1988 Nov 5;204(1):109-27. doi: 10.1016/0022-2836(88)90603-1.

Abstract

Arrays of nucleosomes were positioned with respect to the GAL1-GAL10 intergenic region inserted into Saccharomyces cerevisiae minichromosomes. Deletions of DNA flanking the upstream activation sequence left the array unaltered, showing that nucleosome positioning was not a consequence of sequence-specific histone-DNA interactions but depended on proximity to the galactose-responsive upstream activation sequence (UASG). Replacement of the upstream activation sequence by synthetic oligonucleotides with different protein-binding properties identified a short sequence within this region that is responsible for the ordered array. This sequence overlaps a binding site for GAL4 protein, a positive regulator of transcription, but exerts its effect on chromatin structure independently of GAL4, probably through binding a novel factor that is not GAL-specific.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Base Sequence
  • Binding Sites
  • Chromatin
  • Chromosomes
  • DNA, Fungal / genetics
  • Fungal Proteins / metabolism*
  • Nucleosomes / metabolism*
  • Regulatory Sequences, Nucleic Acid
  • Saccharomyces cerevisiae / genetics*
  • Saccharomyces cerevisiae / metabolism

Substances

  • Chromatin
  • DNA, Fungal
  • Fungal Proteins
  • Nucleosomes