Biosynthesis, characterization and antibacterial activity of silver nanoparticles using an endophytic fungal supernatant of Raphanus sativus

Tej Singh; Kumari Jyoti; Amar Patnaik; Ajeet Singh; Ranchan Chauhan; S S Chandel

doi:10.1016/j.jgeb.2017.04.005

Biosynthesis, characterization and antibacterial activity of silver nanoparticles using an endophytic fungal supernatant of Raphanus sativus

J Genet Eng Biotechnol. 2017 Jun;15(1):31-39. doi: 10.1016/j.jgeb.2017.04.005. Epub 2017 Apr 22.

Authors

Tej Singh¹, Kumari Jyoti², Amar Patnaik³, Ajeet Singh², Ranchan Chauhan⁴, S S Chandel⁵

Affiliations

¹ Department of Mechanical Engineering, Manav Bharti University, Solan 173229, India.
² Department of Biotechnology, G.B.P.E.C., Pauri Garhwal, 246194, India.
³ Department of Mechanical Engineering, M.N.I.T. Jaipur, Rajasthan 302017, India.
⁴ Faculty of Engineering and Technology, Shoolini University, Solan 173229, India.
⁵ Department of Applied Sciences, Manav Bharti University, Solan 173229, India.

Abstract

In this study, biological synthesis of silver nanoparticles (AgNPs) from supernatant of endophytic fungus Alternaria sp. isolated from the healthy leaves of Raphanus sativus is studied. The synthesized AgNPs are characterized using UV-vis spectroscopy and Fourier transform-infrared spectroscopy (FTIR). The structural analysis is done by powder X-ray diffraction (XRD) method. The stability of AgNPs is studied by dynamic light scattering (DLS) method. The size and shape of AgNPs are observed by transmission electron microscopy (TEM) and atomic force microscopy (AFM) and found to be spherical with an average particles size of 4-30 nm. Further, these AgNPs have been found to be highly toxic against human pathogenic bacteria, suggesting the possibility of using AgNPs as efficient antibacterial agents.

Keywords: AFM; AgNPs; Alternaria sp.; Antibacterial activity; Raphanus sativus; TEM.