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, 15 (1), 127-137

Microbial Diversity Structure in Acetate Single Chamber Microbial Fuel Cell for Electricity Generation

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Microbial Diversity Structure in Acetate Single Chamber Microbial Fuel Cell for Electricity Generation

Dena Z Khater et al. J Genet Eng Biotechnol.

Abstract

This study investigates the performance of acetate feed membrane less single chamber microbial fuel cell and physical characterization of the bio film present on the anode surface using Scanning Electron Microscope (SEM) and 16S rRNA analyzer. The performance has been investigated using Teflon treated carbon paper with 0.3 mg/cm2 Pt/C loaded as a cathode and carbon paper as an anode. The maximum open circuit potential is noticed as 791 mV, the system successfully revealed a maximum power density of 86.1 mW m-2 at stable current density of 354 mA m-2 with high coulombic efficiency of 65% at maximum degradation rate of 96%. SEM showed the dense adherence of microorganisms on the anode. 16S rRNA sequencing results indicates phylogenetic mixture in the communities of anodic biofilm and there is no single dominant bacterial species. The dominant phyla are Firmicutes, Gamma Proteobacteria, Alpha Proteobacteria, Actinobacteria, with ten dominant microbial strains: Bacillus firmus, Shewanella profunda, Bacillus isronensis, Brevundimonas bullata, Pseudomonas putida, Planococcus citreus, Micrococcus endophyticus, Acinetobacter tandoii, Bacillus safensis and Shewanella xiamenensis.

Keywords: 16S rRNA; Acetate; Biofilm; Columbic efficiency; Microbial fuel cell; SEM.

Figures

Fig. 1
Fig. 1
Voltage versus time curve for acetate at no load.
Fig. 3
Fig. 3
Change of COD with time behavior at external resistance load (550 Ω).
Fig. 2
Fig. 2
Performances of the mediator less single chamber microbial fuel cell operated under acetate fed batch mode at 550 Ω (blue line) and 1000 Ω (a) Potential, (b) Current density, and (c) Power density vs. time.
Fig. 6
Fig. 6
(a) Phylum distributions for taxonomically assigned from the anode biofilm of MFC. (b) Bacterial community profile of single chamber MFC inoculated with aerobic sludge with acetate. Amplified Samples from the anodic bacterial communities are analyzed by denaturing gradient gel electrophoresis (DGGE) of PCR-amplified genes coding for 16S rRNA.
Fig. 4
Fig. 4
Steady-state power and polarization curves of acetate MSCMFC (a) Polarization curve and (b) Power curve.
Fig. 5
Fig. 5
SEM images (a & b) for carbon paper- free anode before adding microbial community at different magnification. SEM images of (c & d) showing morphological characters of acetate oxidizing bacteria under different magnifications.

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