Abstract
This SOP describes a closed system for isolating GMP-grade CD4+CD25+ T cells from non-mobilized leukapheresis collections (nMLCs), independent of a clean room in a certified GMP premises, by using CliniMACS format GMP grade reagents (CD25-labeled magnetic beads with/without pre-depletion of CD8+ T cells and CD19+ B cells), a GMP grade-A laminar hood and CliniMACS cell processing system.
Keywords:
CD25; CliniMACS; GMP; Leukapheresis collection; Magnetic beads.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Antigens, CD19 / immunology
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B-Lymphocytes / immunology
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CD4 Antigens / immunology
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CD8-Positive T-Lymphocytes / immunology
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Cell Separation / methods*
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Humans
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Immunomagnetic Separation / methods*
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Interleukin-2 Receptor alpha Subunit / immunology
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Leukapheresis / methods*
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T-Lymphocytes / cytology*
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T-Lymphocytes / immunology
Substances
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Antigens, CD19
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CD4 Antigens
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Interleukin-2 Receptor alpha Subunit