An Innovative Standard Operation Procedure for Isolating GMP-Grade CD4+CD25+ T Cells from Non-Mobilized Leukapheresis

Methods Mol Biol. 2019:1899:67-83. doi: 10.1007/978-1-4939-8938-6_6.

Abstract

This SOP describes a closed system for isolating GMP-grade CD4+CD25+ T cells from non-mobilized leukapheresis collections (nMLCs), independent of a clean room in a certified GMP premises, by using CliniMACS format GMP grade reagents (CD25-labeled magnetic beads with/without pre-depletion of CD8+ T cells and CD19+ B cells), a GMP grade-A laminar hood and CliniMACS cell processing system.

Keywords: CD25; CliniMACS; GMP; Leukapheresis collection; Magnetic beads.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Antigens, CD19 / immunology
  • B-Lymphocytes / immunology
  • CD4 Antigens / immunology
  • CD8-Positive T-Lymphocytes / immunology
  • Cell Separation / methods*
  • Humans
  • Immunomagnetic Separation / methods*
  • Interleukin-2 Receptor alpha Subunit / immunology
  • Leukapheresis / methods*
  • T-Lymphocytes / cytology*
  • T-Lymphocytes / immunology

Substances

  • Antigens, CD19
  • CD4 Antigens
  • Interleukin-2 Receptor alpha Subunit