A Real-Time Multiplex PCR Assay for Detection of Elizabethkingia Species and Differentiation between Elizabethkingia anophelis and E. meningoseptica

J Clin Microbiol. 2019 Mar 28;57(4):e01619-18. doi: 10.1128/JCM.01619-18. Print 2019 Apr.


Nosocomial infections of Elizabethkingia species can have fatal outcomes if not identified and treated properly. The current diagnostic tools available require culture and isolation, which can extend the reporting time and delay treatment. Using comparative genomics, we developed an efficient multiplex real-time PCR for the simultaneous detection of all known species of Elizabethkingia, as well as differentiating the two most commonly reported species, Elizabethkingia anophelis and Elizabethkingia meningoseptica.

Keywords: Elizabethkingia; differential assay; molecular diagnostics; real-time PCR.

MeSH terms

  • DNA, Bacterial / isolation & purification
  • Flavobacteriaceae / classification*
  • Flavobacteriaceae / isolation & purification*
  • Flavobacteriaceae Infections / microbiology
  • Genome, Bacterial
  • Genomics*
  • Humans
  • Molecular Diagnostic Techniques / methods*
  • Multiplex Polymerase Chain Reaction / methods*
  • Phylogeny
  • Sensitivity and Specificity
  • Sequence Analysis, DNA


  • DNA, Bacterial

Supplementary concepts

  • Elizabethkingia anophelis