Generation of Definitive Neural Progenitor Cells from Human Pluripotent Stem Cells for Transplantation into Spinal Cord Injury

Methods Mol Biol. 2019;1919:25-41. doi: 10.1007/978-1-4939-9007-8_3.

Abstract

In this chapter, we first describe two interchangeable protocols optimized in our lab for deriving definitive neuronal progenitor cells from human pluripotent stem cells (hPSCs). The resultant NPCs can then be propagated and differentiated to produce differing proportions of neurons, oligodendrocytes, and astrocytes as required for in vitro cell culture studies or in vivo transplantation. Following these protocols, we explain the method for transplanting these cells into the rat model of spinal cord injury (SCI).

Keywords: Cell transplantation; Neural progenitor cells; Pluripotent stem cells; Spinal cord injury.

MeSH terms

  • Animals
  • Cell Culture Techniques
  • Cell Differentiation*
  • Cells, Cultured
  • Disease Models, Animal
  • Embryoid Bodies
  • Humans
  • Neural Stem Cells / cytology*
  • Neural Stem Cells / metabolism
  • Pluripotent Stem Cells / cytology*
  • Pluripotent Stem Cells / metabolism
  • Rats
  • Receptors, Notch / metabolism
  • Spinal Cord Injuries / therapy
  • Stem Cell Transplantation*

Substances

  • Receptors, Notch