Generation of Definitive Neural Progenitor Cells from Human Pluripotent Stem Cells for Transplantation into Spinal Cord Injury

doi:10.1007/978-1-4939-9007-8_3

. 2019:1919:25-41.

doi: 10.1007/978-1-4939-9007-8_3.

Generation of Definitive Neural Progenitor Cells from Human Pluripotent Stem Cells for Transplantation into Spinal Cord Injury

Mohamad Khazaei¹, Christopher S Ahuja^{1

2

3}, Christopher E Rodgers^{1

2}, Priscilla Chan^{1

2}, Michael G Fehlings^{4

5

6

7

8}

Affiliations

¹ Division of Genetics and Development, Krembil Research Institute, Toronto, ON, Canada.
² Institute of Medical Science, University of Toronto, Toronto, ON, Canada.
³ Division of Neurosurgery, University of Toronto, Toronto, ON, Canada.
⁴ Division of Genetics and Development, Krembil Research Institute, Toronto, ON, Canada. Michael.Fehlings@uhn.ca.
⁵ Institute of Medical Science, University of Toronto, Toronto, ON, Canada. Michael.Fehlings@uhn.ca.
⁶ Division of Neurosurgery, University of Toronto, Toronto, ON, Canada. Michael.Fehlings@uhn.ca.
⁷ Spinal Program, Toronto Western Hospital, University Health Network, Toronto, ON, Canada. Michael.Fehlings@uhn.ca.
⁸ Faculty of Medicine, University of Toronto, Toronto, ON, Canada. Michael.Fehlings@uhn.ca.

PMID: 30656619
DOI: 10.1007/978-1-4939-9007-8_3

Generation of Definitive Neural Progenitor Cells from Human Pluripotent Stem Cells for Transplantation into Spinal Cord Injury

Mohamad Khazaei et al. Methods Mol Biol. 2019.

. 2019:1919:25-41.

doi: 10.1007/978-1-4939-9007-8_3.

Authors

Mohamad Khazaei¹, Christopher S Ahuja^{1

2

3}, Christopher E Rodgers^{1

2}, Priscilla Chan^{1

2}, Michael G Fehlings^{4

5

6

7

8}

Affiliations

¹ Division of Genetics and Development, Krembil Research Institute, Toronto, ON, Canada.
² Institute of Medical Science, University of Toronto, Toronto, ON, Canada.
³ Division of Neurosurgery, University of Toronto, Toronto, ON, Canada.
⁴ Division of Genetics and Development, Krembil Research Institute, Toronto, ON, Canada. Michael.Fehlings@uhn.ca.
⁵ Institute of Medical Science, University of Toronto, Toronto, ON, Canada. Michael.Fehlings@uhn.ca.
⁶ Division of Neurosurgery, University of Toronto, Toronto, ON, Canada. Michael.Fehlings@uhn.ca.
⁷ Spinal Program, Toronto Western Hospital, University Health Network, Toronto, ON, Canada. Michael.Fehlings@uhn.ca.
⁸ Faculty of Medicine, University of Toronto, Toronto, ON, Canada. Michael.Fehlings@uhn.ca.

PMID: 30656619
DOI: 10.1007/978-1-4939-9007-8_3

Abstract

In this chapter, we first describe two interchangeable protocols optimized in our lab for deriving definitive neuronal progenitor cells from human pluripotent stem cells (hPSCs). The resultant NPCs can then be propagated and differentiated to produce differing proportions of neurons, oligodendrocytes, and astrocytes as required for in vitro cell culture studies or in vivo transplantation. Following these protocols, we explain the method for transplanting these cells into the rat model of spinal cord injury (SCI).

Keywords: Cell transplantation; Neural progenitor cells; Pluripotent stem cells; Spinal cord injury.

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Generation of Definitive Neural Progenitor Cells from Human Pluripotent Stem Cells for Transplantation into Spinal Cord Injury

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Generation of Definitive Neural Progenitor Cells from Human Pluripotent Stem Cells for Transplantation into Spinal Cord Injury

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