Identification of Antibacterial Phenolics in Selected Plant Species from Mexican Cloud Forest by Mass Spectrometry Dereplication

Chem Biodivers. 2019 Apr;16(4):e1800603. doi: 10.1002/cbdv.201800603. Epub 2019 Mar 19.


Fifteen plant species from a protected cloud forest (CF) in Veracruz, Mexico, were screened for their in vitro capacity to inhibit the growth of the phytopathogenic bacteria Chryseobacterium sp., Pseudomonas cichorii, Pectobacterium carotovorum and Pantoea stewartii, causal agents of damage to crops like 'chayote', lettuce, potato and corn. As a result, the bioactivity of Turpinia insignis and Leandra cornoides is reported for the first time against Chryseobacterium sp. and P. cichorii. In addition, 24 and 18 compounds not described for these species were dereplicated by an UPLC/MS-MS method, respectively. The identified compounds included simple phenols, hydroxycinnamic acids, flavonoids and coumarins. The antibacterial assay of 12 of them demonstrated the bacteriostatic effect of vanillin, trans-cinnamic acid, scopoletin and umbelliferone against Chryseobacterium sp. These findings confirm for the first time the value of the CF plants from Veracruz as sources of bioactive natural products with antimicrobial properties against phytopathogenic bacteria.

Keywords: Turpinia insignis, Leandra cornoides; antibacterial activity; biological activity; cloud forest plants; phenolics dereplication.

MeSH terms

  • Anti-Bacterial Agents / chemistry
  • Anti-Bacterial Agents / isolation & purification
  • Anti-Bacterial Agents / pharmacology*
  • Chryseobacterium / drug effects
  • Dose-Response Relationship, Drug
  • Magnoliopsida / chemistry*
  • Mass Spectrometry
  • Melastomataceae / chemistry*
  • Mexico
  • Microbial Sensitivity Tests
  • Molecular Structure
  • Pantoea / drug effects
  • Pectobacterium / drug effects
  • Phenols / chemistry
  • Phenols / isolation & purification
  • Phenols / pharmacology*
  • Pseudomonas / drug effects
  • Species Specificity
  • Structure-Activity Relationship


  • Anti-Bacterial Agents
  • Phenols