High-throughput ChIPmentation: freely scalable, single day ChIPseq data generation from very low cell-numbers

BMC Genomics. 2019 Jan 18;20(1):59. doi: 10.1186/s12864-018-5299-0.


Background: Chromatin immunoprecipitation coupled to sequencing (ChIP-seq) is widely used to map histone modifications and transcription factor binding on a genome-wide level.

Results: We present high-throughput ChIPmentation (HT-ChIPmentation) that eliminates the need for DNA purification prior to library amplification and reduces reverse-crosslinking time from hours to minutes.

Conclusions: The resulting workflow is easily established, extremely rapid, and compatible with requirements for very low numbers of FACS sorted cells, high-throughput applications and single day data generation.

Keywords: ChIP-seq; ChIPmentation; Chromatin immunoprecipitation; Epigenetics; High-throughput genomics.

MeSH terms

  • Cell Count
  • Cell Line, Tumor
  • Chromatin Immunoprecipitation / methods*
  • DNA / genetics
  • DNA / metabolism
  • Gene Library*
  • High-Throughput Nucleotide Sequencing / methods*
  • Histone Code
  • Humans
  • Protein Binding
  • Protein Processing, Post-Translational*


  • DNA