Dietary Fructose and Microbiota-Derived Short-Chain Fatty Acids Promote Bacteriophage Production in the Gut Symbiont Lactobacillus reuteri

Cell Host Microbe. 2019 Feb 13;25(2):273-284.e6. doi: 10.1016/j.chom.2018.11.016. Epub 2019 Jan 15.

Abstract

The mammalian intestinal tract contains a complex microbial ecosystem with many lysogens, which are bacteria containing dormant phages (prophages) inserted within their genomes. Approximately half of intestinal viruses are derived from lysogens, suggesting that these bacteria encounter triggers that promote phage production. We show that prophages of the gut symbiont Lactobacillus reuteri are activated during gastrointestinal transit and that phage production is further increased in response to a fructose-enriched diet. Fructose and exposure to short-chain fatty acids activate the Ack pathway, involved in generating acetic acid, which in turn triggers the bacterial stress response that promotes phage production. L. reuteri mutants of the Ack pathway or RecA, a stress response component, exhibit decreased phage production. Thus, prophages in a gut symbiont can be induced by diet and metabolites affected by diet, which provides a potential mechanistic explanation for the effects of diet on the intestinal phage community.

Keywords: Lactobacillus reuteri; SCFA; diet; fructose; gut symbiont; phage; prophage; sugar.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Fatty Acids, Volatile / metabolism*
  • Fructose / metabolism*
  • Gastrointestinal Tract / microbiology*
  • Gastrointestinal Tract / virology*
  • Lactobacillus reuteri / growth & development
  • Lactobacillus reuteri / metabolism*
  • Lactobacillus reuteri / virology*
  • Male
  • Metabolic Networks and Pathways / drug effects
  • Mice, Inbred C57BL
  • Prophages / growth & development*
  • Stress, Physiological
  • Virus Activation

Substances

  • Fatty Acids, Volatile
  • Fructose