An online resource for GPCR structure determination and analysis

Nat Methods. 2019 Feb;16(2):151-162. doi: 10.1038/s41592-018-0302-x. Epub 2019 Jan 21.


G-protein-coupled receptors (GPCRs) transduce physiological and sensory stimuli into appropriate cellular responses and mediate the actions of one-third of drugs. GPCR structural studies have revealed the general bases of receptor activation, signaling, drug action and allosteric modulation, but so far cover only 13% of nonolfactory receptors. We broadly surveyed the receptor modifications/engineering and methods used to produce all available GPCR crystal and cryo-electron microscopy (cryo-EM) structures, and present an interactive resource integrated in GPCRdb ( ) to assist users in designing constructs and browsing appropriate experimental conditions for structure studies.

Publication types

  • Research Support, Non-U.S. Gov't
  • Review

MeSH terms

  • Allosteric Site
  • Animals
  • Cattle
  • Computational Biology / methods*
  • Cryoelectron Microscopy
  • Crystallography, X-Ray
  • Databases, Protein
  • Drug Design
  • Glycosylation
  • HEK293 Cells
  • Humans
  • Internet*
  • Mutation
  • Phosphorylation
  • Protein Domains
  • Protein Engineering
  • Receptors, G-Protein-Coupled / genetics*
  • Rhodopsin / chemistry
  • Signal Transduction
  • Software


  • Receptors, G-Protein-Coupled
  • Rhodopsin