Metabolic and proteomic mechanism of benzo[a]pyrene degradation by Brevibacillus brevis

Ecotoxicol Environ Saf. 2019 May 15;172:1-10. doi: 10.1016/j.ecoenv.2019.01.044. Epub 2019 Jan 18.

Abstract

Benzo[a]pyrene (BaP) is a model compound of polycyclic aromatic hydrocarbons. The relationship between its toxicity and some target biomolecules has been investigated. To reveal the interactions of BaP biodegradation and metabolic network, BaP intermediates, proteome, carbon metabolism and ion transport were analyzed. The results show that 76% BaP was degraded by Brevibacillus brevis within 7 d through the cleavage of aromatic rings with the production of 1-naphthol and 2-naphthol. During this process, the expression of xylose isomerase was induced for xylose metabolism, whereas, α-cyclodextrin could no longer be metabolized. Lactic acid, acetic acid and oxalic acid at 0.1-1.2 mg dm-3 were released stemming from their enhanced biosynthesis in the pathways of pyruvate metabolism and citrate cycle, while 5-7 mg dm-3 of PO43- were transported for energy metabolism. The relative abundance of 43 proteins was significantly increased for pyruvate metabolism, citrate cycle, amino acid metabolism, purine metabolism, ribosome metabolism and protein synthesis.

Keywords: Benzo[a]pyrene; Biodegradation; Enzyme; Ion; Proteomics.

MeSH terms

  • Bacillus / metabolism
  • Benzo(a)pyrene / chemistry
  • Benzo(a)pyrene / metabolism*
  • Biodegradation, Environmental
  • Brevibacillus / metabolism*
  • Carbon / metabolism
  • Energy Metabolism
  • Naphthols / chemistry
  • Naphthols / metabolism
  • Proteome / metabolism
  • Proteomics

Substances

  • Naphthols
  • Proteome
  • 1-naphthol
  • Benzo(a)pyrene
  • Carbon
  • 2-naphthol