Potential of Coffee Fruit Extract and Quinic Acid on Adipogenesis and Lipolysis in 3T3-L1 Adipocytes

Kobe J Med Sci. 2018 Oct 15;64(3):E84-E92.

Abstract

This study was to assess the impact of different colors of coffee fruit (green, yellow and red) on adipogenesis and/or lipolysis using 3T3-L1 adipocytes. Characterization of chemical constituents in different colors of coffee fruit extracts was determined by ESI-Q-TOF-MS. The cytotoxicity of the extracts in 3T3-L1 preadipocytes were evaluated by MTT assay. Oil-red O staining and amount of glycerol released in 3T3-L1 adipocytes were measured for lipid accumulation and lipolysis activity. All coffee fruit extracts displayed similar chromatographic profiles by chlorogenic acid > caffeoylquinic acid > caffeic acid. Different colors of raw coffee fruit possessed inhibitory adipogenesis activity in 3T3-L1 adipocytes, especially CRD decreased lipid accumulation approximately 47%. Furthermore, all extracts except CYF and their major compounds (malic, quinic, and chlorogenic acid) increased glycerol release. Our data suggest that different colors of coffee fruit extract have possessed anti-adipogenic and lipolytic properties and may contribute to the anti-obesity effects.

Keywords: adipogenesis; chlorogenic acid; coffee; lipolysis; quinic acid.

MeSH terms

  • 3T3-L1 Cells
  • Adipocytes / drug effects*
  • Adipocytes / metabolism*
  • Adipogenesis / drug effects*
  • Animals
  • Anti-Obesity Agents / chemistry
  • Anti-Obesity Agents / isolation & purification
  • Anti-Obesity Agents / pharmacology
  • Coffea / chemistry
  • Coffee / chemistry*
  • Fruit / chemistry
  • Lipolysis / drug effects*
  • Mice
  • Pigmentation
  • Plant Extracts / chemistry
  • Plant Extracts / pharmacology
  • Quinic Acid / pharmacology*

Substances

  • Anti-Obesity Agents
  • Coffee
  • Plant Extracts
  • Quinic Acid