Molecular cloning and sequence analysis of a developmentally regulated cysteine-rich outer membrane protein from Chlamydia trachomatis

Gene. 1988 Nov 30;71(2):307-14. doi: 10.1016/0378-1119(88)90047-9.

Abstract

Two overlapping genomic fragments have been cloned from Chlamydia trachomatis serovar L1 DNA. Sequence determination of 2530 bp has revealed two open reading frames coding for 'cysteine-rich' (Cr) proteins. One of these proteins was confirmed, by analysis of the inferred amino acid sequence, as the 60-kDa Cr outer membrane protein associated with differentiation of reticulate bodies (RBs) into elementary bodies (EBs). The other smaller 15-kDa protein contained a high percentage of methionine and cysteine and may correspond to a reported smaller and co-ordinately synthesised Cr outer-membrane protein also associated with RB to EB differentiation. Sequencing showed three potential stem-loop structures within the 5', 3' and intergenic regions of the cloned fragment. Southern-blot analysis revealed that the cloned fragment is conserved in ten serovars of C. trachomatis and that a strongly cross-hybridising fragment is also present in Chlamydia psittaci.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Bacterial Outer Membrane Proteins / genetics*
  • Base Sequence
  • Chlamydia trachomatis / genetics*
  • Cloning, Molecular*
  • Cysteine / analysis*
  • DNA / genetics
  • Escherichia coli / genetics
  • Genes, Bacterial
  • Molecular Sequence Data
  • Nucleic Acid Hybridization

Substances

  • Bacterial Outer Membrane Proteins
  • DNA
  • Cysteine

Associated data

  • GENBANK/M23180