Anti-ulcerogenic activity of dentatin from clausena excavata Burm.f. against ethanol-induced gastric ulcer in rats: Possible role of mucus and anti-oxidant effect

Heyam Mohamed Ali Sidahmed; Jamuna Vadivelu; Mun Fai Loke; Ismail Adam Arbab; Bustamam Abdul; Mohd Aspollah Sukari; Siddig Ibrahim Abdelwahab

doi:10.1016/j.phymed.2018.06.036

Anti-ulcerogenic activity of dentatin from clausena excavata Burm.f. against ethanol-induced gastric ulcer in rats: Possible role of mucus and anti-oxidant effect

Phytomedicine. 2019 Mar 1:55:31-39. doi: 10.1016/j.phymed.2018.06.036. Epub 2018 Aug 3.

Authors

Heyam Mohamed Ali Sidahmed¹, Jamuna Vadivelu², Mun Fai Loke², Ismail Adam Arbab³, Bustamam Abdul³, Mohd Aspollah Sukari³, Siddig Ibrahim Abdelwahab⁴

Affiliations

¹ Department of Medical Microbiology, Faculty of Medicine, University of Malaya, 50603 Kuala Lumpur, Malaysia. Electronic address: diamondhm.mohamed@gmail.com.
² Department of Medical Microbiology, Faculty of Medicine, University of Malaya, 50603 Kuala Lumpur, Malaysia.
³ Department of Chemistry, Faculty of Science, Universiti Putra Malaysia UPM, 43400 Serdang, Selangor, Malaysia.
⁴ Substance Abuse Research Centre, Jazan University, P.O.Box 114, Jazan, Saudi Arabia.

PMID: 30668441
DOI: 10.1016/j.phymed.2018.06.036

Abstract

Background: Clausena excavata Burm.f. (Rutaceae) has been used for the treatment of stomach disorders including peptic ulcer.

Purpose: In this study, we aimed to investigate dentatin isolated from C. excavata Burm.f., for anti-ulcer activity against ethanol ulcer model in rats.

Methods: Gastric acid output, ulcer index, serum profile, histological evaluation using Hematoxylin and eosin (HE), periodic acid Schiff base stainings and immunohistochemical localization for heat shock proteins 70 (HSP70) were all investigated. Possible involvement of reduced glutathione (GSH), lipid peroxidation, prostaglandin E₂ (PGE₂), superoxide dismutase (SOD) enzymes, radical scavenging, and anti-Helicobacter pylori activity were investigated.

Results: Dentatin showed anti-secretory activity against the pylorus ligature model and protected the gastric mucosa from ethanol ulceration, as revealed by the improved macroscopic and histological appearance. Dentatin significantly increased the gastric homogenate content of PGE₂ GSH and SOD. Dentatin inhibited the lipid peroxidation as revealed by the reduced gastric content of malondialdehyde (MDA). Moreover, dentatin up-regulated HSP70 expression. However, dentatin showed insignificant anti-H. pylori activity.

Conclusion: Dentatin possesses gastro-protective activity, which could be attributed to the anti-secretory, mucus production, anti-oxidant, and HSP70 activities.

Keywords: Gastric ulcer; Heat shock protein 70; Reactive oxygen species.

MeSH terms

Animals
Anti-Ulcer Agents / pharmacology*
Antioxidants / pharmacology*
Clausena / chemistry
Ethanol / adverse effects
Female
Gastric Mucosa / drug effects
Gastric Mucosa / metabolism
Glutathione / metabolism
HSP70 Heat-Shock Proteins / metabolism
Helicobacter pylori / drug effects
Heterocyclic Compounds, 3-Ring / pharmacology*
Lipid Peroxidation / drug effects
Male
Malondialdehyde / metabolism
Mucus / drug effects
Rats, Sprague-Dawley
Stomach Ulcer / chemically induced
Stomach Ulcer / drug therapy*
Stomach Ulcer / pathology
Superoxide Dismutase / metabolism

Substances

Anti-Ulcer Agents
Antioxidants
HSP70 Heat-Shock Proteins
Heterocyclic Compounds, 3-Ring
dentatin
Ethanol
Malondialdehyde
Superoxide Dismutase
Glutathione