Assembly and early maturation of large subunit precursors

RNA. 2019 Apr;25(4):465-471. doi: 10.1261/rna.069799.118. Epub 2019 Jan 22.

Abstract

The eukaryotic ribosome is assembled through a complex process involving more than 200 factors. As preribosomal RNA is transcribed, assembly factors bind the nascent pre-rRNA and guide its correct folding, modification, and cleavage. While these early events in the assembly of the small ribosomal subunit have been relatively well characterized, assembly of the large subunit precursors, or pre-60S, is less well understood. Recent structures of nucleolar intermediates of large subunit assembly have shed light on the role of many early large subunit assembly factors, but how these particles emerge is still unknown. Here, we use the expression and purification of truncated pre-rRNAs to examine the initial assembly of pre-60S particles. Using this approach, we can recapitulate the early recruitment of large subunit assembly factors mainly to the domains I, II, and VI of the assembling 25S rRNA.

Keywords: RNA; biochemistry; ribosome biogenesis.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Aptamers, Nucleotide / chemical synthesis
  • Aptamers, Nucleotide / metabolism
  • Cloning, Molecular
  • Organelle Biogenesis*
  • Plasmids / chemistry
  • Plasmids / metabolism
  • RNA Precursors / genetics*
  • RNA Precursors / metabolism
  • RNA, Ribosomal / genetics*
  • RNA, Ribosomal / metabolism
  • Ribosomal Proteins / genetics*
  • Ribosomal Proteins / metabolism
  • Ribosome Subunits, Large, Eukaryotic / genetics*
  • Ribosome Subunits, Large, Eukaryotic / metabolism
  • Ribosome Subunits, Large, Eukaryotic / ultrastructure
  • Saccharomyces cerevisiae / genetics*
  • Saccharomyces cerevisiae / metabolism
  • Staining and Labeling / methods

Substances

  • Aptamers, Nucleotide
  • RNA Precursors
  • RNA, Ribosomal
  • Ribosomal Proteins
  • RNA, ribosomal, 25S