Expression of MF alpha 1 in MATa cells supersensitive to alpha-factor leads to self-arrest

Mol Gen Genet. 1988 Sep;214(1):85-8. doi: 10.1007/BF00340184.

Abstract

MATa cells of Saccharomyces cerevisiae defective in both the SST1 and SST2 gene products exhibit self-arrest when they express the MF alpha 1 gene under the control of the GAL1 promoter. This response to endogenously produced pheromone can be alleviated by mutations which prevent the production of, or response to, alpha-factor. Suppressors of the self-arrest phenotype include a class of mutants which remain responsive to low levels of pheromone, but are resistant to high levels of alpha-factor. One of these mutants has been mapped to chromosome X, 31 cM distal to SUP4, and defines a new locus designated STE18.

MeSH terms

  • Crosses, Genetic
  • Diploidy
  • Gene Expression Regulation*
  • Genes, Fungal*
  • Mating Factor
  • Mutation
  • Peptide Biosynthesis
  • Peptides / genetics*
  • Peptides / physiology
  • Promoter Regions, Genetic
  • Saccharomyces cerevisiae / genetics*
  • Saccharomyces cerevisiae / growth & development
  • Transformation, Genetic

Substances

  • Peptides
  • Mating Factor