In vitro evaluation of biological activities of Orobanche crenata Forssk. leaves extract

Nat Prod Res. 2020 Nov;34(22):3234-3238. doi: 10.1080/14786419.2018.1552697. Epub 2019 Jan 23.

Abstract

Orobanche crenata Forssk. (Orobanchaceae) is a holoparasitic plant noxious to legumes. Orobanchaceae are well known in traditional medicine for their hypotensive, antihyperglycemic, antispasmodic, analgesic and antimicrobial effects. In this work, the biological activities of the acetonic leaves extract were evaluated. Qualitative chemical analysis was determined through thin layer chromatography. Total phenols and flavonoids content was obtained by the Folin-Ciocalteu and the Aluminium chloride colorimetric methods, respectively. The antimicrobial activity was performed by the broth microdilution method. The extract showed antibacterial activity, particularly against Gram-positive strains, with MIC ranging from 376.00 to 3011.00 µg/ml. Besides, it exhibited antifungal effects on Candida spp. with MIC ranging from 94.10 to 3011.00 µg/ml. In addition, O. crenata inhibited superoxide anion formation and showed a DPPH quenching capacity, particularly at 80 µg/ml. Interestingly, our results are promising thus indicating the importance of O. crenata as a significant source of biologically active natural compounds.

Keywords: Orobanche crenata; antimicrobial activity; antioxidant activity; polyphenols.

MeSH terms

  • Anti-Bacterial Agents / pharmacology
  • Antifungal Agents / pharmacology
  • Antioxidants / chemistry
  • Antioxidants / pharmacology
  • Candida / drug effects
  • Flavonoids / analysis
  • Gram-Negative Bacteria / drug effects
  • Microbial Sensitivity Tests
  • Orobanche / chemistry*
  • Phenols / analysis
  • Plant Extracts / chemistry*
  • Plant Extracts / pharmacology*
  • Plant Leaves / chemistry
  • Superoxides / chemistry
  • Superoxides / metabolism

Substances

  • Anti-Bacterial Agents
  • Antifungal Agents
  • Antioxidants
  • Flavonoids
  • Phenols
  • Plant Extracts
  • Superoxides