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, 141 (5), 1833-1837

Enzyme-Mediated Intercellular Proximity Labeling for Detecting Cell-Cell Interactions

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Enzyme-Mediated Intercellular Proximity Labeling for Detecting Cell-Cell Interactions

Yun Ge et al. J Am Chem Soc.

Abstract

Cell-cell interactions and communications play fundamental roles in life processes but remain largely uncharacterized. We developed an enzyme-mediated proximity cell labeling (EXCELL) strategy as a general method to detect and record cell-cell interactions under living conditions. EXCELL relies on an evolved Staphylococcus aureus transpeptidase sortase A variant (mgSrtA) capable of promiscuous labeling of various cell surface proteins containing a monoglycine residue at the N-terminus. Displaying mgSrtA on the surface of a cell of interest allows the labeling and detection of interacting cells in a proximity-dependent fashion.

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