Anticancer Effect of Cisplatin-Loaded Poly (Butylcyanoacrylate) Nanoparticles on A172 Brain Cancer Cells Line

Asian Pac J Cancer Prev. 2019 Jan 25;20(1):303-309. doi: 10.31557/APJCP.2019.20.1.303.


Background: Drug delivery systems have been designed to achieve targeted delivery and control the release rate of the drugs. A serious challenge associated with drug delivery systems is the presence of the blood-brain barrier which limits drugs penetration. In the current study, the effects of cisplatin nanoparticles on A172 brain cancer cell line were investigated. Methods: Cisplatin nanoparticles were produced by miniemulsion polymerization technique and their properties were evaluated. Drug release assay was performed to characterize the nanoparticles’ properties. Here, we examined the effects of cisplatin nanoparticles and free form of cisplatin on A172 cancer cell line. MTT assay was performed for different concentrations of the drug. To measure the apoptosis rate in A172 cell line in the presence of cisplatin nanoparticles or its free from, Annexin V staining method was used. Results: Our results indicated that loading type of cisplatin was physical loading and only 4.7% of cisplatin was released after 68 h. Furthermore, MTT assay showed that cisplatin nanoparticles in all concentrations had more cytotoxic effects on the cells comparing with the free form of cisplatin and control groups. We also showed that cisplatin nanoparticles could increase apoptosis in cancer cells more than the drug in the free form by using flow cytometry technique. Conclusion: Overall, these findings proved that cisplatin loaded on poly (Butylcyanoacrylate) nanoparticles, was more efficient than the free form of cisplatin in treating A172 cancer cell line.

Keywords: Cisplatin; poly (Butylcyanoacrylate) nanoparticles; apoptosis; A172 cells.

MeSH terms

  • Antineoplastic Agents / chemistry
  • Antineoplastic Agents / pharmacology*
  • Apoptosis / drug effects
  • Brain Neoplasms / drug therapy*
  • Cell Line, Tumor
  • Cisplatin / chemistry
  • Cisplatin / pharmacology*
  • Drug Carriers / chemistry
  • Drug Delivery Systems / methods
  • Enbucrilate / administration & dosage
  • Enbucrilate / chemistry*
  • Humans
  • Nanoparticles / administration & dosage*
  • Nanoparticles / chemistry
  • Polymers / administration & dosage
  • Polymers / chemistry


  • Antineoplastic Agents
  • Drug Carriers
  • Polymers
  • Enbucrilate
  • Cisplatin