Down-regulation of Cdk1 activity in G1 coordinates the G1/S gene expression programme with genome replication

Curr Genet. 2019 Jun;65(3):685-690. doi: 10.1007/s00294-018-00926-y. Epub 2019 Jan 24.


Cell division is regulated by cyclin-dependent kinases (Cdks) and requires the periodic activation and inactivation of transcription factors that generate waves of gene expression in different cell-cycle phases. In fission yeast, the MCB-binding transcription factor (MBF) is activated at the end of G1 and regulates the expression of a set of genes that encode for proteins involved in the G1/S transition and DNA replication. Here, we review the importance of controlling MBF by Cdk activity at the onset of S phase. Furthermore, we emphasize that MBF regulation by Cdk is particularly critical under conditions in which G1 is extended, such as in nitrogen-poor environments, where down-regulation of Cdk activity in G1 is crucial to generate a proper wave of MBF-dependent transcription at the end of G1, which is critical to promote a successful S phase.

Keywords: CDK inhibition; Cell cycle; G1/S transcription; Gad8; Genomic instability; MBF; Nitrogen; Rum1; Ste9; TORC2; dNTPs.

Publication types

  • Review

MeSH terms

  • CDC2 Protein Kinase / genetics
  • CDC2 Protein Kinase / metabolism*
  • DNA Replication*
  • G1 Phase*
  • Gene Expression Regulation, Fungal*
  • Genome, Fungal*
  • S Phase*
  • Schizosaccharomyces / genetics*
  • Schizosaccharomyces / metabolism
  • Schizosaccharomyces pombe Proteins / genetics
  • Schizosaccharomyces pombe Proteins / metabolism


  • Schizosaccharomyces pombe Proteins
  • CDC2 Protein Kinase