Lack of lamins A and C in mammalian hemopoietic cell lines devoid of intermediate filament proteins

Eur J Cell Biol. 1988 Oct;47(1):121-31.


Using immunofluorescence microscopy and immunoblot analysis, we have examined the composition of the nuclear lamina in several murine and human cell lines. Whereas it was shown that intermediate filament-positive Ehrlich ascites tumor and HeLa-S3 cells contain the three major mammalian lamin subspecies, only lamin B could be detected in several myeloid- and lymphoid-derived cell lines representative of distinct stages in hemopoietic differentiation but all devoid of cytoplasmic intermediate filament proteins. These included the murine plasmacytoma cell types MPC-11 and MOPC-31C, murine myeloma cells X63-Ag8.6.5.3 and human promyelocytic leukemia cells HL-60. Our results provide the first evidence that mammalian somatic cells capable of normal proliferation may lack both cytoplasmic intermediate filament proteins and a normal complement of nuclear lamins.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cell Line
  • Fluorescent Antibody Technique
  • Gene Expression Regulation
  • Humans
  • Immunoblotting
  • Intermediate Filament Proteins / physiology
  • Intermediate Filament Proteins / ultrastructure*
  • Lamin Type B
  • Lamins
  • Mice
  • Microscopy, Electron
  • Nuclear Proteins / analysis*
  • Nuclear Proteins / physiology
  • Tumor Cells, Cultured / analysis
  • Tumor Cells, Cultured / ultrastructure


  • Intermediate Filament Proteins
  • Lamin Type B
  • Lamins
  • Nuclear Proteins