Developmental changes in interkinetic nuclear migration dynamics with respect to cell-cycle progression in the mouse cerebral cortex ventricular zone

J Comp Neurol. 2019 Jul 1;527(10):1545-1557. doi: 10.1002/cne.24641. Epub 2019 Feb 6.


We have examined the relationship between interkinetic nuclear migration (INM) and cell-cycle progression of apical progenitors in the ventricular zone (VZ) at different stages of mouse cerebral corticogenesis. We report stage-specific changes in INM due to a significant alteration of the nuclear apical movement dynamics with respect to cell-cycle phases. While at early stages, the apical nuclear movement corresponds to the G2 phase, cell-cycle phase specific immunostaining and real-time imaging of PCNA expressing apical progenitors revealed that at midcorticogenesis, the nuclear apical movement is initiated well before the entry into G2, during S phase. We observed that the S phase and G2 phase segments of the nuclear apical movement exhibit different velocities. Experimental shortening of cell-cycle duration via cyclin E overexpression in APs at midcorticogenesis leads to congruent INM behavior changes. This suggests that INM dynamics are under cell-cycle related constraints.

Keywords: RRID:AB_10979488; RRID:AB_2160651; RRID:AB_2534023; RRID:AB_2534096; RRID:AB_2535804; RRID:AB_2535849; RRID:IMSR_CRL:612; RRID:SCR_001905; RRID:SCR_003070; RRID:SCR_008520; cell-cycle; cortical development; cyclin E; interkinetic nuclear migration.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cell Cycle / physiology*
  • Cell Nucleus*
  • Cerebral Cortex / embryology*
  • Mice
  • Neural Stem Cells*
  • Neurogenesis / physiology*