The binding of monomeric amyloid β peptide to serum albumin is affected by major plasma unsaturated fatty acids

Biochem Biophys Res Commun. 2019 Mar 5;510(2):248-253. doi: 10.1016/j.bbrc.2019.01.081. Epub 2019 Jan 23.


Human serum albumin (HSA) serves as a natural depot of amyloid β peptide (Aβ). Improvement of Aβ binding to HSA should impede Alzheimer's disease (AD). We developed a method for quantitation of the interaction between monomeric Aβ40/42 and HSA using surface plasmon resonance spectroscopy. The dissociation constant of HSA complex with recombinant Aβ40/42 is 0.2-0.3 μM. Flemish variant of Aβ40 has 2.5-10-fold higher affinity to HSA. The parameters of the HSA-Aβ interaction are selectively sensitive to HSA binding of major plasma unsaturated fatty acids and Cu2+. Linoleic and arachidonic acids promote the HSA-Aβ42 interaction. The developed methodology for quantitation of HSA-Aβ interaction may serve as a tool for search of compounds favoring HSA-Aβ interaction, thereby preventing AD progression.

Keywords: Alzheimer's disease; Amyloid β peptide; Human serum albumin; Surface plasmon resonance; Unsaturated fatty acid.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Alzheimer Disease / blood
  • Alzheimer Disease / genetics
  • Amyloid beta-Peptides / chemistry*
  • Amyloid beta-Peptides / genetics
  • Amyloid beta-Protein Precursor / chemistry*
  • Amyloid beta-Protein Precursor / genetics
  • Calmodulin / chemistry
  • Disease Progression
  • Fatty Acids, Unsaturated / blood*
  • Humans
  • Ligands
  • Mutation*
  • Parvalbumins / chemistry
  • Peptide Fragments / chemistry*
  • Peptide Fragments / genetics
  • Protein Binding
  • Recombinant Proteins / chemistry
  • Serum Albumin, Human / chemistry*
  • Surface Plasmon Resonance


  • APP protein, human
  • Amyloid beta-Peptides
  • Amyloid beta-Protein Precursor
  • Calmodulin
  • Fatty Acids, Unsaturated
  • Ligands
  • Parvalbumins
  • Peptide Fragments
  • Recombinant Proteins
  • amyloid beta-protein (40-42)
  • Serum Albumin, Human