Requirement of Cellular Protein CCT7 for the Replication of Fowl Adenovirus Serotype 4 (FAdV-4) in Leghorn Male Hepatocellular Cells Via Interaction With the Viral Hexon Protein

Viruses. 2019 Jan 27;11(2):107. doi: 10.3390/v11020107.

Abstract

Fowl adenovirus serotype 4 (FAdV-4) causes hepatitis-hydropericardium syndrome (HHS), leading to severe economic losses in the poultry industry. Although the pathogenesis of FAdV-4 infection has caused much attention, the underlying molecular mechanisms remain poorly understood. Here, we identified chaperonin containing TCP-1 subunit eta (CCT7) as an interacting partner of the FAdV-4 capsid protein hexon. We found that ectopic expression of CCT7 in leghorn male hepatocellular (LMH) cells enhanced hexon expression in pRK5-flag-hexon transfected cells. On the contrary, knockdown of cellular CCT7 by RNAi markedly reduced hexon expression in FAdV-4-infected cells and suppressed viral replication. These data suggest that CCT7 is required for FAdV-4 replication and may serve as a potential target for controlling FAdV-4 infection.

Keywords: CCT7; FAdV-4; Hexon; Replication.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adenoviridae / genetics
  • Adenoviridae / physiology*
  • Adenoviridae Infections / veterinary*
  • Animals
  • Capsid Proteins / genetics
  • Capsid Proteins / metabolism*
  • Chaperonin Containing TCP-1 / genetics*
  • Chaperonin Containing TCP-1 / metabolism
  • Chickens
  • Gene Knockdown Techniques
  • Host Microbial Interactions*
  • Liver / cytology
  • Liver / virology
  • Male
  • Phylogeny
  • Poultry Diseases / virology
  • RNA Interference
  • Serogroup
  • Virus Replication*

Substances

  • Capsid Proteins
  • hexon capsid protein, Adenovirus
  • Chaperonin Containing TCP-1